Transcriptome analysis of Populus x canadensis 'Zhongliao1' in response to low temperature stress

文献类型： 外文期刊

第一作者： Yang, Chengchao

作者： Yang, Chengchao;Li, Xiaoyu;Zhang, Yan;Jin, Hua

作者机构：

关键词： Poplar; Freeze-thaw injury; Overwintering; Low temperature stress; Cold stress response

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundLow temperatures are known to limit the growth and geographical distribution of poplars. Although some transcriptomic studies have been conducted to explore the response of poplar leaves to cold stress, only a few have comprehensively analyzed the effects of low temperature on the transcriptome of poplars and identified genes related to cold stress response and repair of freeze-thaw injury.ResultsWe exposed the Euramerican poplar Zhongliao1 to low temperatures; after stems were exposed to - 40celcius, 4celcius, and 20celcius, the mixture of phloem and cambium was collected for transcriptome sequencing and bioinformatics analysis. A total of 29,060 genes were detected, including 28,739 known genes and 321 novel genes. Several differentially expressed genes (n = 36) were found to be involved in the Ca2+ signaling pathway, starch-sucrose metabolism pathway, abscisic acid signaling pathway, and DNA repair. They were functionally annotated; glucan endo-1,3-beta-glucosidase and UDP-glucuronosyltransferase genes, for instance, showed a close relationship with cold resistance. The expression of 11 differentially expressed genes was verified by qRT-PCR; RNA-Seq and qRT-PCR data were found to be consistent, which validated the robustness of our RNA-Seq findings. Finally, multiple sequence alignment and evolutionary analysis were performed, the results of which suggested a close association between several novel genes and cold resistance in Zhongliao1.ConclusionWe believe that the cold resistance and freeze-thaw injury repair genes identified in this study are of great significance for cold tolerance breeding.

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