Autochthonous bioaugmentation strategies for the successful bioremediation of high-salt petrochemical wastewater using a biosurfactant-producing Enterobacter cloacae Z11

文献类型： 外文期刊

第一作者： Zhang, Ning

作者： Zhang, Ning;Zhang, Ruichang;Zhou, Xinquan;Wei, Xuefeng;Ren, Xing;Zhou, Hanghai;Deng, Zhaochao;Zhang, Chunfang

作者机构：

关键词： Petrochemical wastewater; Autochthonous bioaugmentation; Biosurfactant; Microbial community; Degradation-related enzymes

期刊名称：JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING （ 影响因子：7.7； 五年影响因子：7.3 ）

ISSN： 2213-2929

年卷期： 2024 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Petrochemical wastewater contains a high concentration of refractory aliphatic and aromatic hydrocarbons. Bioremediation of these hazardous materials using autochthonous bioaugmentation (ABA) strategy is attracting increasing interest. Herein, an indigenous biosurfactant-producing bacteria Z11 was isolated and identified as Enterobacter sp. The reintroduction of Z11 into petrochemical wastewater led to a substantial reduction (P < 0.01) in TPHs, decreasing from 8099 to 1723 mg/L, as well as a significant decrease in COD, decreasing from 8064.2 to 1689.2 mg/L. At a critical micelle concentration of 200 mg/L, the biosurfactant synthesized by Z11 exhibited an extraordinary decrease in surface tension from 67.5 to 29 mN/m. The biosurfactant demonstrated favorable surface activity over a wide pH, temperature, and salinity range. Based on a series of chemical analyses, the biosurfactant was classified as phospholipid. The autochthonous bioaugmentation technique developed by Z11 indicated a significant increase (P < 0.05) in the degradation of n-alkanes of varying chain lengths and polycyclic aromatic hydrocarbons. The analysis of the microbial composition demonstrated that the addition of Z11 caused a rise in bacterial populations capable of hydrocarbon decomposition, such as Pseudomonas sp. and Stenotrophomonas sp. The activities of two enzymes involved in degradation, alkane hydroxylase and alcohol dehydrogenase, increased dramatically and showed a significant (P < 0.05) correlation with Enterobacter sp. Z11. The proposed mechanism could be elucidated that functional genes regulate the secretion of biodegradation enzymes and biosurfactants to promote the deep degradation of petroleum hydrocarbons. This study provides technical support for the reintroduction of autochthonous microorganisms capable of producing biosurfactants in implementing in situ ABA strategies.

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