Identification of a Novel Linear B-Cell Epitope in the M Protein of Avian Infectious Bronchitis Coronaviruses

文献类型： 外文期刊

第一作者： Xing, Junji

作者： Xing, Junji;Liu, Shengwang;Han, Zongxi;Shao, Yuhao;Li, Huixin;Kong, Xiangang

作者机构：

关键词： avian infectious bronchitis virus;monoclonal antibody;linear B-cell epitope;epitope mapping;membrane protein;coronavirus

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： This report describes the identification of a novel linear Bell epitope at the C-terminus of the membrane (M) protein of avian infectious bronchitis virus (IBV). A monoclonal antibody (MAb) (designated as 15E2) against the IBV M protein was prepared and a series of 14 partially-overlapping fragments of the IBV M gene were expressed with a GST tag. These peptides were subjected to enzyme-linked immunosorbent as- say (ELISA) and western blotting analysis using MAb 15E2 to identify the epitope. A linear motif, ~(199)FATFVYAK~(206), which was located at the C-terminus of the M protein, was identified by MAb 15E2. ELISA and western blotting also showed that this epitope could be recognized by IBV-positive serum from chicken. Given that 15E2 showed reactivity with the ~(199)FATFVYAK~(206) motif, expressed as a GST fusion protein, in both western blotting_and in an ELISA, we proposed that this motif represented a linear Bell epitope of the M protein. The~(199) FATFVYAK~(206) motif was the minimal requirement for reactivity as dem?onstrated by analysis of the reactivity of 15E2 with several truncated peptides that were derived from the motif. Alignment and comparison of the 15E2-defined epitope sequence with the sequences of other corona-viruses indicated that the epitope is well conserved among chicken and turkey coronaviruses. The identified epitope should be useful in clinical applications and as a tool for the further study of the structure and function of the M protein of IBV.

分类号： Q93

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