Use of CRISPR-Cas tools to engineer Trichoderma species

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Chen, Hongyu;Gong, Ming;Wu, Yingying;Bao, Dapeng;Zou, Gen;Ma, Liang

作者机构：

期刊名称：MICROBIAL BIOTECHNOLOGY （ 影响因子：6.575； 五年影响因子：7.412 ）

ISSN： 1751-7915

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收录情况： SCI

摘要： Given their lignocellulose degradability and biocontrol activities, fungi of the ubiquitously distributed genus Trichoderma have multiple industrial and agricultural applications. Genetic manipulation plays a valuable role in tailoring novel engineered strains with enhanced target traits. Nevertheless, as applied to fungi, the classic tools of genetic manipulation tend to be time-consuming and tedious. However, the recent development of the CRISPR-Cas system for gene editing has enabled researchers to achieve genome-wide gene disruptions, gene replacements, and precise editing, and this technology has emerged as a primary focus for novel developments in engineered strains of Trichoderma. Here, we provide a brief overview of the traditional approaches to genetic manipulation, the different strategies employed in establishing CRSIPR-Cas systems, the utilization of these systems to develop engineered strains of Trichoderma for desired applications, and the future trends in biotechnology.

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