Two evolutionarily duplicated domains individually and post-transcriptionally control SWEET expression for phloem transport

文献类型： 外文期刊

第一作者： Zhang, Chen

作者： Zhang, Chen;Li, Ye;Wang, Jiang;Xue, Xueyi;Beuchat, Gabriel;Chen, Li-Qing;Li, Ye

作者机构：

关键词： coevolution; phloem parenchyma; post-transcriptional regulation; specific expression; SWEET transporter; vascular plants

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.152； 五年影响因子：10.476 ）

ISSN： 0028-646X

年卷期： 2021 年 232 卷 4 期

页码：

收录情况： SCI

摘要： Cell type-specific gene expression is critical for the specialized functions within multicellular organisms. In Arabidopsis, SWEET11 and SWEET12 sugar transporters are specifically expressed in phloem parenchyma (PP) cells and are responsible for sucrose efflux from the PP, the first step of a two-step apoplasmic phloem-loading strategy that initiates the long-distance transport of sugar from leaves to nonphotosynthetic sink tissues. However, we know nothing about what determines the PP cell-specific expression of these SWEETs. Sequence deletions, histochemical beta-glucuronidase (GUS) analysis, cross-sectioning, live-cell imaging, and evolutionary analysis were used to elucidate domains responsible for PP specificity, while a Forster resonance energy transfer (FRET) sensor-based transport assay was used to determine whether substrate specificity coevolved with PP specificity. We identified two domains in the Arabidopsis SWEET11 coding sequence that, along with its promoter (including 5 ' UTR), regulate PP-specific expression at the post-transcriptional level, probably involving RNA-binding proteins. This mechanism is conserved among vascular plants but independent of transport substrate specificity. We conclude that two evolutionarily duplicated coding sequence domains are essential and individually sufficient for PP-specific expression of SWEET11. We also provide a crucial experimental tool to study PP physiology and development.

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