Bacillus lipopeptides inhibit lipase activity and promote 3T3-L1 preadipocyte differentiation

文献类型： 外文期刊

第一作者： Chen, Meichun

作者： Chen, Meichun;Chen, Deju;Xiao, Rongfeng;Zheng, Xuefang;Liu, Bo;Wang, Jieping

作者机构：

关键词： Lipopeptide; lipase inhibitory activity; PPAR gamma

期刊名称：JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY （ 影响因子：5.4； 五年影响因子：4.9 ）

ISSN： 1475-6366

年卷期： 2024 年 39 卷 1 期

页码：

收录情况： SCI

摘要： Bacillus lipopeptides have been reported to display anti-obesity effects. In the present study, Lipopeptides from Bacillus velezensis FJAT-45028 that consisted of iturin, fengycin and surfactin were reported. The lipopeptides exhibited a strong lipase inhibition activity in a concentration-dependent manner with a half maximal inhibitory concentration of 0.012 mg/mL, and the inhibition mechanism and type were reversible and competitive, respectively. Results of CCK8 assay showed that 3T3-L1 preadipocyte cells were completely viable under treatment of 0.050-0.2 mg/mL lipopeptides for 24 or 48 h. It was found that the lipopeptides could increase lipid droplets in the differentiated 3T3-L1 adipocytes in tested concentration and suppress the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma). These results indicated the potential anti-obesity mechanism of the tested lipopeptides might be to inhibit lipase activity but not to suppress lipid accumulation in the adipocytes. Moreover, the lipopeptides could elevate glucose utilisation by 14.43%-33.81% in the differentiated 3T3-L1 adipocytes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序