Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress

文献类型： 外文期刊

第一作者： Shabbir, Samina

作者： Shabbir, Samina;Wang, Weiting;Chen, Mao;Liu, Panting;Dai, Yonghua;Sun, Lingling;Gou, Qiyu;Liu, Renbin;Hu, Guoquan;He, Mingxiong;Shabbir, Samina;Wang, Weiting;Chen, Mao;Wu, Bo;Liu, Panting;Dai, Yonghua;Sun, Lingling;Gou, Qiyu;Liu, Renbin;Hu, Guoquan;Nawaz, Mohsin;Boruah, Prerona;Kulyar, Muhammad Fakhar-e-Alam;Younis, Tahira;He, Mingxiong

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关键词： Acetic acid; Furfural; Zymomonas mobilis; Lignocellulosic biomass; RNA-Seq; Proteomics

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：6.4； 五年影响因子：6.3 ）

ISSN：

年卷期： 2023 年 22 卷 1 期

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收录情况： SCI

摘要： Acetic acid and furfural (AF) are two major inhibitors of microorganisms during lignocellulosic ethanol production. In our previous study, we successfully engineered Zymomonas mobilis 532 (ZM532) strain by genome shuffling, but the molecular mechanisms of tolerance to inhibitors were still unknown. Therefore, this study investigated the responses of ZM532 and its wild-type Z. mobilis (ZM4) to AF using multi-omics approaches (transcriptomics, genomics, and label free quantitative proteomics). Based on RNA-Seq data, two differentially expressed genes, ZMO_RS02740 (up-regulated) and ZMO_RS06525 (down-regulated) were knocked out and over-expressed through CRISPR-Cas technology to investigate their roles in AF tolerance. Overall, we identified 1865 and 14 novel DEGs in ZM532 and wild-type ZM4. In contrast, 1532 proteins were identified in ZM532 and wild-type ZM4. Among these, we found 96 important genes in ZM532 involving acid resistance mechanisms and survival rates against stressors. Furthermore, our knockout results demonstrated that growth activity and glucose consumption of mutant strains ZM532 increment ZMO_RS02740 and ZM4 increment ZMO_RS02740 decreased with increased fermentation time from 42 to 55 h and ethanol production up to 58% in ZM532 than that in ZM532 increment ZMO_RS02740. Hence, these findings suggest ZMO_RS02740 as a protective strategy for ZM ethanol production under stressful conditions.

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