Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development

文献类型： 外文期刊

第一作者： Huang, Quan-Sheng

作者： Huang, Quan-Sheng;Wang, Hai-Yun;Gao, Peng;Xia, Gui-Xian;Huang, Quan-Sheng;Wang, Guo-Ying;Wang, Hai-Yun;Gao, Peng;Xia, Gui-Xian;Huang, Quan-Sheng

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期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

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收录情况： SCI

摘要： The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.

分类号： Q942

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