Improved detection sensitivity of anti-PRV variant antibodies through preparation of anti-gB and anti-gE monoclonal antibodies and development of blocking ELISAs

文献类型： 外文期刊

第一作者： Guo, Zhenyang

作者： Guo, Zhenyang;Xu, Hu;Zhang, Siyu;Kang, Haonan;Li, Chao;Sun, Qi;Zhao, Jing;Li, Jinhao;Zhou, Guohui;Wang, Qian;Xiang, Lirun;Tang, Yandong;Liu, Huairan;An, Tongqing;Cai, Xuehui;Tian, Zhijun;Zhang, Hongliang;Peng, Jinmei;Leng, Chaoliang

作者机构：

关键词： Pseudorabies virus; Monoclonal antibody and B -cell epitope; Blocking ELISA

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 260 卷

页码：

收录情况： SCI

摘要： Since 2011, PRV has resurged in China and is characterized by a mutated strain with significant alterations in antigenicity and virulence. Therefore, we hypothesized that antibody detection kits based on classic PRV strains may have limitations in detecting PRV variants. For more sensitive antibody detection of PRV variants, two MABs targeting the gB and gE proteins were developed. IFA revealed that these MABs exhibited strong reactivity toward both classic and variant PRV strains. MAB-gE recognizes a novel conserved linear B -cell epitope (41PSAEVWD47), while MAB-gB recognizes a conformational B -cell epitope. The binding of both MABs was effectively inhibited in the PRV-positive pig blood samples. Accordingly, we established blocking-ELISAs to detect anti-PRV gB and gE antibodies, which achieved higher sensitivity than commercial kits. Moreover, the clinical serum samples results of our method and that of IFA were in high agreement, and our test results had a higher coincidence rate than that of a commercial kit. Assessing antibody levels by our methods at various times following immunization and challenge accurately reflected the trend of antibody -level changes and revealed the conversion to positive antibody status before the commercial kit. Our method is crucial for monitoring PRV infections, assessing immune responses, and controlling disease.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Identification of a linear B-cell epitope on the "puff" loop of the Senecavirus A VP2 protein involved in receptor binding

    作者：Zhou, Hanrong;Sun, Mingxia;Su, Shibo;Meng, Liang;Shi, Xinqi;Li, Xin;Wang, Haiwei;Ma, Hongwei;Cai, Xuehui;Tang, Yan-Dong;An, Tongqing;Meng, Fandan;Yang, Wei;Yang, Lan;Ma, Hongwei;Cai, Xuehui;An, Tongqing

    关键词：Senecavirus A (SVA); B-cell epitopes (BCEs); monoclonal antibodies (mAbs); VP2 protein; receptor binding

  • Metagenomic analysis of individual mosquito viromes reveals the geographical patterns and drivers of viral diversity

    作者：Pan, Yuan-Fei;Gou, Qin-Yu;Luo, Geng-Yan;Le, Shi-Jia;Xin, Gen-Yang;Wang, Jing;Hou, Xin;Peng, Min-Wu;Kong, Jian-Bin;Chen, Xin-Xin;Yang, Chun-Hui;Mei, Shi-Qiang;Liao, Yu-Qi;Cheng, Jing-Xia;Guo, Deyin;Wu, Wei-Chen;Shi, Mang;Pan, Yuan-Fei;Li, Bo;Pan, Yuan-Fei;Gou, Qin-Yu;Luo, Geng-Yan;Le, Shi-Jia;Xin, Gen-Yang;Wang, Jing;Hou, Xin;Peng, Min-Wu;Kong, Jian-Bin;Chen, Xin-Xin;Yang, Chun-Hui;Mei, Shi-Qiang;Liao, Yu-Qi;Cheng, Jing-Xia;Wu, Wei-Chen;Shi, Mang;Zhao, Hailong;Shi, Pei-Bo;Ren, Zirui;Peng, Shiqin;Jin, Xin;Wang, Daxi;Li, Junhua;Zhao, Hailong;Shi, Pei-Bo;Ren, Zirui;Peng, Shiqin;Wang, Daxi;Li, Junhua;Shi, Pei-Bo;Tian, Jun-Hua;Feng, Yun;Yang, Wei-Hong;Wang, Juan;Li, Kun;Wu, De;Tang, Guangpeng;Zhang, Bing;Chaolemen;Wu, Yu-Hui;Wang, Jian-Bo;An, Tongqing;Huang, Xinyi;Eden, John-Sebastian;Eden, John-Sebastian;Holmes, Edward C.;Li, Jun;Guo, Deyin;Liang, Guodong;Li, Bo;Li, Bo

    关键词：

  • Metagenomic analysis of individual mosquito viromes reveals the geographical patterns and drivers of viral diversity

    作者：Pan, Yuan-Fei;Gou, Qin-Yu;Luo, Geng-Yan;Le, Shi-Jia;Xin, Gen-Yang;Wang, Jing;Hou, Xin;Peng, Min-Wu;Kong, Jian-Bin;Chen, Xin-Xin;Yang, Chun-Hui;Mei, Shi-Qiang;Liao, Yu-Qi;Cheng, Jing-Xia;Guo, Deyin;Wu, Wei-Chen;Shi, Mang;Pan, Yuan-Fei;Li, Bo;Pan, Yuan-Fei;Gou, Qin-Yu;Luo, Geng-Yan;Le, Shi-Jia;Xin, Gen-Yang;Wang, Jing;Hou, Xin;Peng, Min-Wu;Kong, Jian-Bin;Chen, Xin-Xin;Yang, Chun-Hui;Mei, Shi-Qiang;Liao, Yu-Qi;Cheng, Jing-Xia;Wu, Wei-Chen;Shi, Mang;Zhao, Hailong;Shi, Pei-Bo;Ren, Zirui;Peng, Shiqin;Jin, Xin;Wang, Daxi;Li, Junhua;Zhao, Hailong;Shi, Pei-Bo;Ren, Zirui;Peng, Shiqin;Wang, Daxi;Li, Junhua;Shi, Pei-Bo;Tian, Jun-Hua;Feng, Yun;Yang, Wei-Hong;Wang, Juan;Li, Kun;Wu, De;Tang, Guangpeng;Zhang, Bing;Chaolemen;Wu, Yu-Hui;Wang, Jian-Bo;An, Tongqing;Huang, Xinyi;Eden, John-Sebastian;Eden, John-Sebastian;Holmes, Edward C.;Li, Jun;Guo, Deyin;Liang, Guodong;Li, Bo;Li, Bo

    关键词：

  • Genomic analyses reveal the stepwise domestication and genetic mechanism of curd biogenesis in cauliflower

    作者：Chen, Rui;Yao, Xingwei;Zhang, Xiaoli;Yang, Yingxia;Lyu, Mingjie;Wang, Qian;Zhang, Guan;Wang, Mengmeng;Li, Yanhao;Duan, Lijin;Xie, Tianyu;Li, Haichao;Yang, Yuyao;Zhang, Hong;Guo, Yutong;Jia, Guiying;Sun, Deling;Chen, Ke;Su, Xiao;Lin, Tao;Chen, Ke;Li, Haichao;Yang, Yuyao;Zhang, Hong;Guo, Yutong;Jia, Guiying;Ge, Xianhong;Sarris, Panagiotis F.;Sarris, Panagiotis F.

    关键词：

  • Genomic Characterization of HLJDZD55: The First L1B PRRSV in China

    作者：Li, Jinhao;Xu, Hu;Li, Chao;Zhao, Jing;Gong, Bangjun;Sun, Qi;Guo, Zhenyang;Zhang, Siyu;Zhang, Menglin;Xiang, Lirun;Tang, Yan-dong;Wu, Jianan;Wang, Qian;Peng, Jinmei;Zhou, Guohui;Liu, Huairan;An, Tongqing;Cai, Xuehui;Tian, Zhi-Jun;Zhang, Hongliang

    关键词：

  • NGS-Based Multi-Allelic InDel Genotyping and Fingerprinting Facilitate Genetic Discrimination in Grapevine (Vitis vinifera L.)

    作者：Jia, Guiying;Zhang, Na;Yang, Yingxia;Zhang, Hong;Guo, Yutong;Wang, Qian;Zhang, He;Chen, Rui;Huang, Jianquan;Lyu, Mingjie;Jia, Guiying;Zhang, Hong;Guo, Yutong;Yang, Yingxia;Jin, Qingdong;Jiang, Jianfu;Wu, Jianjin

    关键词：grapevine; multi-allelic InDels; next-generation sequencing; fingerprinting; population structure

  • QTL detection and candidate gene identification of qCTB1 for cold tolerance in the Yunnan plateau landrace rice

    作者：Guo, Haifeng;Zou, Andong;Khan, Najeeb Ullah;Gu, Yunsong;Li, Jin;Sun, Xingming;Zhang, Zhanying;Zhang, Hongliang;Li, Jinjie;Li, Zichao;Guo, Yongmei;Li, Huahui;Wu, Zhigang;Yuan, Pingrong;Zeng, Yawen;Guo, Haifeng;Peng, Youliang

    关键词：Rice; Cold tolerance; QTL analysis; QTL pyramiding; Candidate gene

微信小程序