Synthetic photorespiratory bypass improves rice productivity by enhancing photosynthesis and nitrogen uptake

文献类型： 外文期刊

第一作者： Chen, Guoxin

作者： Chen, Guoxin;Li, Yanni;Jin, Kaining;Gao, Jiabei;Wu, Suting;Cui, Xuean;Lu, Tiegang;Zhang, Zhiguo;Jin, Kaining;Mao, Chuanzao

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.1 ）

ISSN： 1040-4651

年卷期： 2025 年 37 卷 1 期

页码：

收录情况： SCI

摘要： Photorespiration, often considered as a wasteful process, is a key target for bioengineering to improve crop yields. Several photorespiratory bypasses have been designed to efficiently metabolize 2-phosphoglycolate and increase the CO2 concentration in chloroplasts, thereby reducing photorespiration. However, the suppression of primary nitrate assimilation remains an issue when photorespiration is inhibited. In this study, we designed a carbon and nitrogen metabolism-coupled photorespiratory bypass, termed the GCBG bypass, in rice (Oryza sativa) chloroplasts. Our results demonstrated efficient assembly and expression of the GCBG bypass in rice chloroplasts, which affected the levels of typical metabolites and their derivatives of natural photorespiration and enhanced the photosynthetic efficiency. Metabolomic analyses revealed that oxaloacetate, produced from glycolate in chloroplasts, positively impacted amino acid synthesis, energy metabolism, and sugar synthesis. The engineered GCBG plants showed an average yield increase of 19.0% (17.8% to 20.2%) compared with wild-type plants under natural growth conditions, alongside improved nitrogen uptake, which compensated for 44.1% of yield losses under nitrogen-limited conditions. In summary, the GCBG bypass substantially improved the photosynthetic efficiency, biomass, and yield in rice by integrating carbon and nitrogen metabolism. This study introduces a strategy for engineering high-yielding rice or other crops with improved photosynthetic efficiency and nitrogen uptake. Carbon and nitrogen metabolism-coupled photorespiratory bypass substantially improved the photosynthetic efficiency, biomass, and yield in rice by integrating carbon and nitrogen metabolism.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序