Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4

文献类型： 外文期刊

第一作者： Chen, Pin

作者： Chen, Pin;Chen, Xiaoqian;Yu, Wei;Zhou, Bo;Du, Peng;Liu, Libo;Li, Chun;Liu, Lihua;Yang, Yuzhuo

作者机构：

关键词： Ciprofloxacin; Lactobacillus plantarum DNZ-4; Key enzymes; Metabolism

期刊名称：FOOD SCIENCE AND HUMAN WELLNESS （ 影响因子：8.022； ）

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年卷期： 2022 年 11 卷 2 期

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收录情况： SCI

摘要： Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, beta-galactosidase and Na+, K+-ATPase after 1/2 minimum bacteriostatic concentration (MIC) CIP treatment were significantly decreased (P < 0.01). Gas chromatography-mass spectrometry was used to analysis the changes of main metabolites in the cells and principal component analysis and partial least square model were constructed. The results indicated that CIP could cause changes in intracellular fatty acids, carbohydrates and amino acids, and the mechanism of amino acid metabolism under CIP stress was significantly inhibited. L. plantarum DNZ-4 made stress response to CIP by regulating the ratio of saturated fatty acids and unsaturated fats. This experiment revealed the changes of growth and metabolism mechanism of L. plantarum DNZ-4 under CIP stress, which help to provide technical means for the development of effective probiotics preparation products. (C) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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