A CCA1-like MYB subfamily member CsMYB128 participates in chilling sensitivity and cold tolerance in tea plants ( Camellia sinensis)

文献类型： 外文期刊

第一作者： Yu, Shuwei

作者： Yu, Shuwei;Zhang, Xiaojia;Gao, Ying;Yuan, Changbo;Liu, Xinyu;Yao, Yuantao;Song, Lubin;Li, Penghui;Liu, Hongjie;Liu, Jiaojiao;Zhao, Jian

作者机构：

关键词： Tea plant; CsCCA1-like MYB; Cold stress

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 294 卷

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收录情况： SCI

摘要： While flavonoid accumulation, light radiation, and cold stress are intrinsically connected in tea plants, yet the underlying mechanisms remain elusive. The circadian protein CCA1 and CCA1-like MYB transcription factors (TFs) play important roles in coordinating light and temperature signals in plant-environment interactions, their homologs in tea plants have not been addressed. Here we analyzed CsCCA1-like MYB subfamily in tea genome and found one member, a circadian gene CsMYB128 responding to cold stress. Antisense knockdown of CsMYB128 in tea buds rendered cold tolerance in cold tolerance tests. Metabolite profiling, yeast hybrid and promoter trans-activation assays further demonstrated that CsMYB128 negatively regulated flavonol biosynthesis by repressing CsFLS1 in flavonol biosynthesis and CsCBF1 in cold tolerance. Given CsCBF1 also activated CsMYB128 transcription, the negative feedback regulation loop indicates a balance between tea plant growth promoted by CsMYB128 and cold tolerance meanwhile growth inhibition by CsCBF1. Moreover, CsICE1 interacted with and inhibited CsMYB128 repressor activity to promote cold tolerance. CsMYB128 is thus characterized as an early cold-responsive gene negatively regulating tea plant cold response and balancing tea plant growth and cold tolerance. This study provides insights into the roles of CCA1-like subfamily MYB TFs in regulating tea plant growth and interactions with environments.

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