Characterization of a novel cry8 gene specific to Melolonthidae pests: Holotrichia oblita and Holotrichia parallela

文献类型: 外文期刊

第一作者: Yan, Guixin

作者: Yan, Guixin;Zhang, Jie;Song, Fuping;Wang, Rongyan;Feng, Shuliang;Huang, Dafang

作者机构:

关键词: Bacillus thuringiensis;Cry8Ga;Holotrichia oblita;Holotrichia parallela;PCR-RFLP

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A new polymerase chain reaction-restriction fragment length polymorphism method for the identification of cry8-type genes from Bacillus thuringiensis has been established by designing a pair of new universal primers. By this method, a novel gene, cry8Ga1, encoding a polypeptide of 1,157 amino acids with a deduced molecular mass of 131.2 kDa was identified and cloned from B. thuringiensis HBF-18. Recombinant B. thuringiensis strain HD8G, harboring cry8Ga1, has insecticidal activity against larvae of Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. This is the first report of a Cry toxin that has insecticidal activity to Melolonthidae pest H. oblita.

分类号: Q939.9

  • 相关文献

[1]Genomic sequencing identifies novel Bacillus thuringiensis Vip1/Vip2 binary and Cry8 toxins that have high toxicity to Scarabaeoidea larvae. Zhang, Yanrui,Shu, Changlong,Song, Fuping,Zhang, Jie,Crickmore, Neil,Wang, Qinglei,Du, Lixin.

[2]Holotrichia oblita Midgut Proteins That Bind to Bacillus thuringiensis Cry8-Like Toxin and Assembly of the H-oblita Midgut Tissue Transcriptome. Huang, Ying,Shu, Changlong,Song, Fuping,Zhang, Jie,Jiang, Jian,Lai, Jinsheng,Jiang, Jian,Lai, Jinsheng,Soberon, Mario,Bravo, Alejandra,Liu, Chunqing.

[3]Assembling of Holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to Cry8Ea toxin from Bacillus thuringiensis. Shu, Changlong,Tan, Shuqian,Yin, Jiao,Geng, Lili,Song, Fuping,Li, Kebin,Zhang, Jie,Soberon, Mario,Bravo, Alejandra,Liu, Chunqing.

[4]Construction of a Bacillus thuringiensis engineered strain with high toxicity and broad pesticidal spectrum against coleopteran insects. Liu, Jingjing,Yan, Guixin,Shu, Changlong,Zhao, Can,Song, Fuping,Zhou, Lin,Ma, Junlan,Zhang, Jie,Liu, Chunqin,Huang, Dafang.

[5]Characterization of one novel cry8 gene from Bacillus thuringiensis strain Q52-7. Liu, Rongmei,Zhang, Qingli,Zhao, Shiyuan,Shao, Gaoxiang,Zhang, Xiaofeng,Gao, Jiguo,Li, Haitao,Shu, Changlong.

[6]Characterization of insecticidal crystal protein cry gene of Bacillus thuringiensis from soil of Sichuan Basin, China and cloning of novel haplotypes cry gene. Zhu, Jun,Tang, Jie,Li, Yunyan,Zheng, Aiping,Li, Ping,Tan, Furong,Zheng, Aiping,Zhu, Jun,Tang, Jie,Li, Yunyan,Li, Ping.

[7]Rapid cloning, identification, and application of one novel crystal protein gene cry30Fa1 from Bacillus thuringiensis. Tan, Furong,Tang, Xueming,Tan, Furong,Zheng, Aiping,Zhu, Jun,Wang, Lingxia,Li, Shuangcheng,Deng, Qiming,Wang, Shiquan,Li, Ping,Zheng, Aiping,Zhu, Jun,Wang, Lingxia,Li, Shuangcheng,Deng, Qiming,Wang, Shiquan,Li, Ping.

[8]Identification of vip3A-type genes from Bacillus thuringiensis strains and characterization of a novel vip3A-type gene. Song, F.,Zhang, J.,Liu, R.,He, K.,Tan, J.,Huang, D.. 2007

[9]Prediction of the key binding site of odorant-binding protein of Holotrichia oblita Faldermann ( Coleoptera: Scarabaeida). Zhuang, X.,Wang, Q.,Wang, B.,Zhong, T.,Cao, Y.,Li, K.,Yin, J.. 2014

[10]Identification and comparison of candidate odorant receptor genes in the olfactory and non-olfactory organs of Holotrichia oblita Faldermann by transcriptome analysis. Wei, Hongshuang,Shu, Changlong,Zhang, Shuai,Cao, Yazhong,Yin, Jiao,Luo, Chen. 2017

[11]Function and Immunocytochemical Localization of Two Novel Odorant-Binding Proteins in Olfactory Sensilla of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae). Deng, Sisi,Yin, Jiao,Zhong, Tao,Cao, Yazhong,Li, Kebin. 2012

[12]A New Type I Peritrophic Membrane Protein from Larval Holotrichia oblita (Coleoptera: Melolonthidae) Binds to Chitin. Liu, Xiaomin,Guo, Wei,Liu, Xiaomin,Guo, Wei,Li, Ruijun,Zhao, Dan,Li, Xinna,Li, Jie. 2014

[13]Influence of Constant Temperature on Reproductive Parameters of Holotrichia oblita (Coleoptera: Scarabaeidae). Chen, Haoliang,Lin, Lulu,Xie, Minghui,Zhang, Guangling,Su, Weihua. 2015

[14]Controlled Release Study on Microencapsulated Mixture of Fipronil and Chlorpyrifos for the Management of White Grubs (Holotrichia parallela) in Peanuts (Arachis hypogaea L.). Yang, Daibin,Yan, Xiaojing,Yuan, Huizhu,Li, Guangxing. 2014

[15]A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela. Geng, Lili,Chi, Jing,Shu, Changlong,Song, Fuping,Zhang, Jie,Gresshoff, Peter M.,Huang, Dafang. 2013

[16]Discovery and characterization of endo-xylanase and beta-xylosidase from a highly xylanolytic bacterium in the hindgut of Holotrichia parallela larvae. Xu, Jing,Zhang, Hongyu,Sheng, Ping,Xu, Jing,Zhang, Hongyu,Saccone, Giuseppe,Li, Kebin. 2014

[17]A new cell line derived from embryonic tissues of Holotrichia parallela (Coleoptera:Scarabaeidae). Li, Miao-Miao,Zheng, Gui-Ling,Su, Rui,Wan, Fang-Hao,Li, Chang-You,Li, Miao-Miao,Wan, Fang-Hao.

[18]Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle. Li, Jianbin,Zhang, Yi,Zhang, Yuan,Li, Jianbin,Wang, Hongmei,Hou, Minghai,Zhong, Jifeng. 2011

[19]Genetic variability in Grapevine virus A from Vitis vinifera L. x Vitis labrusca L. in Sichuan, China. Xi, Dehui,Yuan, Shu,Lin, Honghui,Wang, Jianhui,Chen, Keling,Li, Hongwen,Liu, Xiao,Wang, Jianhui,Liu, Jianjun,Chen, Keling,Li, Hongwen,Liu, Xiao,Liu, Jianjun,Ercisli, Sezai. 2012

[20]The Polymorphism of a Mutation of IGF-1 Gene on Two Goat Breeds in China. Wang Mou,Yu Hui,Huang Yong-Zhen,Xin Jing-Jing,Xin Ya-Ping,Chen Hong,Liu Wu-Jun,Fang Yi,Huang Xi-Xia,Yao Xin-Kui,Yu Shi-Gang,Fang Guang-Xin,Tian Ke-Chuan,Chen Hong. 2010

作者其他论文 更多>>