N-acetylcysteine ameliorate cytotoxic injury in piglets sertoli cells induced by zearalenone and deoxynivalenol

文献类型： 外文期刊

第一作者： Cao, Li

作者： Cao, Li;Zhao, Jie;Xu, Jingru;Zhu, Lei;Rahman, Sajid Ur;Feng, Shibin;Li, Yu;Wu, Jinjie;Wang, Xichun;Li, Yu;Wu, Jinjie;Wang, Xichun;Rahman, Sajid Ur

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关键词： Zearalenone; Deoxynivalenol; Sertoli cells; N-acetylcysteine; Oxidative damage; Apoptosis

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

年卷期： 2021 年 28 卷 42 期

页码：

收录情况： SCI

摘要： Zearalenone (ZEA) and Deoxynivalenol (DON) are two mycotoxins highly detected in agricultural products and feed. Both mycotoxins produce reproductive toxicity and pose a serious threat to human and animal health, among which pigs are the most sensitive animals. Sertoli cells (SCs) play an important role in spermatogenesis; however, the combined toxicity of ZEA and DON and the screening of effective protective agents remains to be determined. By studying the effects of N-acetylcysteine (NAC) on the cells exposed to 20 mu M of ZEA and 0.6 mu M of DON, we explored the protective mechanism of NAC (4 mM) on the cytotoxic injury of piglets SCs induced by both mycotoxins. The results showed that the combination of ZEA and DON destroy organdies and SCs structures, NAC significantly alleviates the damage caused by ZEA and DON. NAC also significantly increased the expression and distribution of zonula occludens 1 (ZO-1), decreased the relative mRNA and protein expression levels of Bax, Bid, caspase-3, and caspase-9, and increased Bcl-2 expression level and inhibited the decrease of mitochondrial membrane potential. Further, NAC also eases the cell cycle arrest and oxidative stress caused by ZEA and DON. In summary, our results show that NAC could alleviate SCs injury via reducing the oxidative damage and apoptosis caused by ZEA and DON.

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