NADPH-cytochrome P450 reductase involved in the lambda-cyhalothrin susceptibility on the green mirid bug Apolygus lucorum

文献类型： 外文期刊

第一作者： Zhen, Congai

作者： Zhen, Congai;Wu, Rui;Zhang, Lei;Tan, Yao;Zhang, Ansheng

作者机构：

关键词： Apolygus lucorum; lambda-cyhalothrin resistance; NADPH-cytochrome P450 reductase

期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.6； 五年影响因子：1.7 ）

ISSN： 0007-4853

年卷期： 2024 年 114 卷 5 期

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收录情况： SCI

摘要： NADPH-cytochrome P450 reductase (CPR) is crucial for the detoxification process catalysed by cytochrome P450, which targets various exogenous xenobiotics, as well as pesticides. In our research, we successfully obtained the complete cDNA sequence of Apolygus lucorum's CPR (AlCPR) using reverse transcription PCR along with rapid amplification of cDNA ends technology. Bioinformatics analysis exhibited that the inferred amino acid sequence of AlCPR is characteristic of standard CPRs, featuring an N-terminal membrane anchor and three conserved FMN, FAD and NADP binding sites. Phylogenetic result revealed that AlCPR was positioned within the Hemiptera cluster, showing a close evolutionary relationship with the CPR of Cimex lectularius. The real-time quantitative PCR results demonstrated widespread expression of AlCPR across various life stages and tissues of A. lucorum, with the most prominent expression in adults and the abdominal region. Injecting double-stranded RNA of AlCPR only significantly increased the lambda-cyhalothrin susceptibility in lambda-cyhalothrin-resistant strain rather than the susceptible strain. These findings suggest a potential link between AlCPR and the P450-dependent defence mechanism against lambda-cyhalothrin in A. lucorum.

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