Spatial and Temporal Expression Characteristics of the HBB Gene Family in Six Different Pig Breeds

文献类型： 外文期刊

第一作者： Guo, Xin

作者： Guo, Xin;Zhang, Jing;Liu, Zhiguo;Mu, Yulian;Huang, Lei;Li, Kui

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关键词： beta-thalassemia; pigs; HBB gene family; hemoglobin

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 10 期

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收录情况： SCI

摘要： beta-Thalassemia is one of the most prevalent inherited diseases in China. It is important to develop animal models to accurately simulate human beta-thalassemia and there are unique advantages to studying beta-thalassemia in pigs. However, there are only few reports on the systematic analysis of the beta-thalassemia-related genes and their expression pattern in pigs so far. Therefore, in this study, we firstly predicted 11 porcine hemoglobin-encoding genes and found that there was no HBG gene in pigs, indicating that the globin switches might not exist in pigs. A new hemoglobin-encoding gene, 'HBB-like', was found in pigs, which showed high conservation in its amino sequences between pigs and humans. Then, we studied the evolutionary relationship of hemoglobin-encoding genes in human, pig and mouse. The results showed that the beta-chain structure of pig and human was highly similar. In addition, we analyzed the hemoglobin-encoding gene expressions by using the iswine database and qPCR. Our results showed significant differences in the spatiotemporal expression patterns among the four genes (HBA, HBB, HBB-like and HBE) in three developmental stages of six different pig breeds. Our study provides an important theoretical basis for further construction of a gene-edited beta-thalassemia miniature pig model to assess beta--thalassemia treatments.

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