Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis

文献类型： 外文期刊

第一作者： Peng, Shi-Qing

作者： Peng, Shi-Qing;Zhu, Jia-Hong;Li, Hui-Liang;Tian, Wei-Min

作者机构：

关键词： Methods and Techniques;Molecular Genetics: Biochemistry and Molecular Biophysics

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

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收录情况： SCI

摘要： The full-length cDNA encoding a cysteine protease, designated HbCP1, was isolated for the first time from Hevea brasiliensis by the rapid amplification of cDNA ends (PACE) method. HbCP1 contained a 1371 bp open reading frame encoding 457 amino acids. The deduced HbCP1 protein, which showed high identity to cysteine proteases of other plant species, was predicted to possess a putative repeat in toxin (RTX) domain at the N-terminal and a granulin (GRAN) domain at the C-terminal. Southern blot analysis indicated that the HbCP1 gene is present as a single copy in the rubber tree. Transcription pattern analysis revealed that HbCP1 had high transcription in laticifer, and low transcription in bark and leaf. The transcription of HbCP1 in latex was induced by ethylene and tapping. Cloning of the HbCP1 gene will enable us to further understand the molecular characterization of cysteine protease and its possible function in the rubber tree.

分类号： Q

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