A Na+/H+-Exchanger Gene from Penaeus monodon: Molecular Characterization and Expression Analysis under Ammonia Nitrogen Stress

文献类型： 外文期刊

第一作者： Li, Yundong

作者： Li, Yundong;Jiang, Shigui;Fan, Hongdi;Yang, Qibin;Jiang, Song;Huang, Jianhua;Yang, Lishi;Zhang, Wenwen;Zhou, Falin;Li, Yundong;Jiang, Shigui;Yang, Qibin;Chen, Xu;Zhou, Falin;Li, Yundong;Zhou, Falin;Li, Yundong

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关键词： NHE; Penaeus monodon; ammonia nitrogen stress; intestine; gill; RNAi

期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.744； 五年影响因子：2.727 ）

ISSN：

年卷期： 2022 年 10 卷 12 期

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收录情况： SCI

摘要： Na+/H+-exchanger (NHE) assumes a significant part in different particle transport in creatures. A clone of Penaeus monodon NHE cDNA was examined in this study (PmNHE), and its impact on high-concentration ammonia nitrogen stress was researched. The 877-amino acid (aa) protein was encoded by a full-length PmNHE cDNA that was 2788 base pairs (bp) long and had a 2643-bp open reading frame (ORF). The findings show that PmNHE was expressed in all of the P. monodon organs that were tested, including the intestine, muscle, hemolymph, heart, hepatopancreas, stomach, epidermis, gill, testis, and ovary, and the intestine and muscle were found to have the highest levels of PmNHE expression. The expression of PmNHE in the gill tissue of P. monodon was significantly up-regulated under high levels of ammonia nitrogen stress. The expression of PmNHE in the intestine of P. monodon under high-concentration ammonia nitrogen stress was significant. When exposed to high concentrations of ammonia nitrogen stress, P. monodon exhibited shorter survival times than the two control groups. Hence, it is suggested in the present study that PmNHE may have a significant impact on the environment with high levels of ammonia nitrogen.

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