Evaluation of the immunoprotective effects of porcine deltacoronavirus subunit vaccines

文献类型： 外文期刊

第一作者： Yu, Ruiming

作者： Yu, Ruiming;Zhang, Liping;Zhou, Peng;Zhang, Zhongwang;Liu, Xiaoqing;Wang, Yonglu;Guo, Huichen;Pan, Li;Liu, Xinsheng;Yu, Ruiming;Zhang, Liping;Liu, Xinsheng

作者机构： Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Lanzhou, Peoples R China;Natl Ctr Technol Innovat Pigs, Chongqing 402460, Peoples R China

关键词： Porcine deltacoronavirus; Protein expression; Subunit vaccine; Immunoprotection

期刊名称：VIROLOGY （ 2022影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2024 年 590 卷

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus (PDCoV), a new porcine enteric coronavirus, has seriously endangered the pig breeding industry and caused great economic losses. However, a PDCoV vaccine is not commercially available. Therefore, new and efficient PDCoV vaccines must be developed without delay. In this study, we used the ExpiCHO eukaryotic expression system to express and purify the following 3 structural proteins of PDCoV: S, N and M. Subsequently, the level of humoral and cellular immunity induced by the S protein (immunization with the S protein alone) and a protein mixture (immunization with a mixture of S, N and M proteins) were evaluated in mice and piglets, respectively, and the performances of the 2 immunizations in a challenge protection test were assessed in piglets. The results showed that both the S protein and the protein mixture induced the production of high levels of specific IgG antibodies and neutralizing antibodies and effectively neutralized PDCoV-infected LLCPK cells in vitro. Furthermore, compared with the S protein, the N and M proteins in the protein mixture promoted the expression of CD8+ T cells and IFN-gamma, induced a stronger cellular immune response, and effectively protected 4/5 of the piglets from PDCoV infection. In conclusion, the results of this study showed that the N and M proteins play important roles in inducing an immunoprotective response. Using N and M antigens as effective antigenic components in the development of PDCoV vaccines in the future will effectively increase the immune efficacy of the vaccines.

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