Glutathione peroxidase LtGPX3 contributes to oxidative stress tolerance, virulence, and plant defense suppression in the peach gummosis fungus Lasiodiplodia theobromae

文献类型： 外文期刊

第一作者： Zhang, He

作者： Zhang, He;Shen, Xingyi;Shen, Wanqi;Zhang, Dongmei;Huang, Xue;Zhu, Kaijie;Liu, Junwei;Li, Guohuai;Zhang, He;Shen, Wanqi

作者机构：

关键词： Fungal virulence; Homologous recombination; Reactive oxygen species; Plant defense response; Prunus persica; Woody tree disease

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.4； 五年影响因子：4.2 ）

ISSN： 2096-5362

年卷期： 2024 年 6 卷 1 期

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收录情况： SCI

摘要： The notorious woody plant-degrading pathogen Lasiodiplodia theobromae is a major causal agent of peach gummosis, one of the prevalent and devastating trunk diseases to peach production; however, its pathogenesis is largely unknown. Our previous study showed that L. theobromae LtGPX3, which encodes a glutathione peroxidase resembling yeast GPX3/HYR1-like, was constantly and dramatically upregulated at the infectious stages. Here, we functionally characterized LtGPX3 using the CRISPR-Cas9-aided split marker approach. The Delta Ltgpx3 deletion mutants displayed increased sensitivity to the osmotic stress agent KCl and less sensitivity to the cell wall-damaging agent calcofluor white. Exogenous oxidants highly induced the expression of LtGPX3, and the Delta Ltgpx3 mutants displayed increased sensitivity to ROS-generating oxidants. Pathogenicity assays revealed that Delta Ltgpx3 mutants showed compromised virulence in peach shoots, which was partially restored when peach shoots were pretreated with an NADPH oxidase inhibitor before inoculation. Moreover, ROS levels were strongly boosted, and transcripts of plant defense-related genes were highly induced in the Delta Ltgpx3 mutants-infected peach shoots compared with the wild-type-inoculated. Overall, our results showed the essential roles of LtGPX3 in the oxidative stress response and tolerance and pathological functions in L. theobromae. These findings deepen our understanding of the survival strategies of the woody plant-degrading pathogen L. theobromae and provide new insights into developing new strategies for peach gummosis disease control.

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