Fine mapping of a major co-localized QTL associated with self-incompatibility identified in two F2 populations (broccoli x cauliflower and cauliflower x Chinese kale)

文献类型： 外文期刊

第一作者： Shen, Yusen

作者： Shen, Yusen;Wang, Jiansheng;Sheng, Xiaoguang;Yu, Huifang;Shaw, Ranjan K.;Song, Mengfei;Cai, Shiyi;Qiao, Shuting;Lin, Fan;Gu, Honghui;Cai, Shiyi;Lin, Fan;Qiao, Shuting

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 12 期

页码：

收录情况： SCI

摘要： Key messageA major QTL responsible for self-incompatibility was stably identified in two F2 populations. Through fine mapping and qRT-PCR analysis, ARK3 emerged as the most promising candidate gene, playing a pivotal role in regulating self-incompatibility in Brassica oleracea.AbstractSelf-incompatibility (SI) is a common phenomenon in Brassica oleracea species, which can maintain genetic diversity but will also limit seed production. Although the S locus has been extensively studied in Arabidopsis and some Brassicaceae crops, map-based cloning of self-incompatibility genes has not been conducted in Brassica oleracea, such as cauliflower and broccoli. In the present study, we identified a major co-localized QTL on chromosome C6 that control SI in two F2 populations derived from intervarietal crosses: broccoli x cauliflower (CL_F2) and cauliflower x Chinese kale (CJ_F2). Subsequently, this QTL was narrowed down to 168.5 Kb through fine mapping using 3,429 F2:3 progenies and 12 available KASP markers. Within this 168.5 Kb region, BolC6t39084H, a homologue of Arabidopsis ARK3, could be a candidate gene that plays a key role in regulating SI in B. oleracea species. This finding can pave the way for an in-depth understanding of the molecular mechanisms underlying SI, and will contribute to the seed production of B. oleracea vegetables.

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