Skin mucus proteomic provides insights into the alkaline tolerance of grass carp (Ctenopharyngodon idella)

文献类型： 外文期刊

第一作者： Li, Chenghao

作者： Li, Chenghao;Hu, Guo;Huang, Jing;Lu, Cuiyun;Zhang, Kexin;Zou, Yuting;Zhang, Limin;Zheng, Xianhu;Chang, Yumei;Shen, Yubang;Zou, Yuting

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关键词： Ctenopharyngodon idella; Proteomics; Alkali-tolerant fish; Skin mucus

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.4； 五年影响因子：2.8 ）

ISSN： 1744-117X

年卷期： 2025 年 55 卷

页码：

收录情况： SCI

摘要： Fish skin mucus is a dynamic mucosal layer located between the epidermis and the environment, serving as the first line of defense against external environmental stresses. Studying changes in fish skin mucus under high alkaline conditions can help identify potential biomarkers of alkaline tolerance, thereby providing a basis for developing non-invasive detection methods. In this study, grass carp (Ctenopharyngodon idella) were subjected to a 7-day stress test at three different alkalinity levels: low alkalinity (10 mmol/L sodium bicarbonate), medium alkalinity (30 mmol/L sodium bicarbonate), and high alkalinity (50 mmol/L sodium bicarbonate). Fish skin mucus was collected and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-independent acquisition (DIA) mode. A total of 9656 proteins were identified across 12 samples from four groups. The low and medium alkalinity groups shared more common functional enrichments, while the high alkalinity group showed significant enrichments in pathways related to oxygen binding, the immune system, and myocarditis. More nodes and pairwise interactions were founded in the high alkalinity group. Multiple proteins related to osmoregulation, including Prostaglandin G/H Synthase 1 (PTGS1) and Group XV Phospholipase A2 (PLA2G15), are highly expressed in the high-alkalinity treatment group. These results suggest that high-alkalinity stress-induced changes in the skin mucus of grass carp reflect the continuous activation of osmoregulatory mechanisms, and the high expression of PTGS1 and PLA2G15 in the skin mucus may serve as signatures of the survival status of grass carp in alkaline conditions.

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