Mutations in the WUSCHEL-related homeobox1 gene cause an increased leaflet number in soybean

文献类型： 外文期刊

第一作者： Li, Yawei

作者： Li, Yawei;Chu, Li;Lyu, Peiyun;Lyu, Wenhuan;Xie, Ping;Zhang, Chunting;Feng, Haoxian;Zhao, Tuanjie;Liu, Bin

作者机构：

关键词： Soybean; GmWOX1A; GmYAB5; Compound leaf development; Seed number per plant

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2024 年 12 卷 6 期

页码：

收录情况： SCI

摘要： WUSCHEL-related homeobox (WOX) transcription factors play a crucial role in lateral organ development in several plant species; however, their precise functions in soybean ( Glycine max [L.] Merr.) were unclear. Here, we identified two independent multi-leaflet soybean mutants, mlw48-8 and mlw48-161, from a CRISPR/Cas9-engineered mutant library in the Williams 82 background. Both mutants exhibited irregular leaf margins, and the upper leaves were narrow and almost lanceolate at maturity. Molecular analysis revealed that these are allelic mutants with independent mutations in the WUSCHEL-related homeobox1 ( GmWOX1A ) gene. A transcriptome analysis demonstrated that GmWOX1A modulates the expression of auxin- and leaf development-related genes. Yeast two-hybrid and split-luciferase complementation imaging assays revealed that GmWOX1A interacts with the YABBY family protein GmYAB5, providing further evidence of its potential involvement in leaf development. Notably, the mlw48-161 mutant showed an increased seed number per plant. Consequently, our study not only provides valuable insights into the role of GmWOX1A in soybean leaf development but also offers a potential strategy for high-yield breeding. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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