Albiflorin ameliorates inflammation and oxidative stress by regulating the NF-Kappa B/NLRP3 pathway in Methotrexate-induced enteritis

文献类型： 外文期刊

第一作者： Zhang, Haihua

作者： Zhang, Haihua;Wang, Jing;Liu, Hongli;Li, Hongqiang;Lang, Wuying;Zhang, Zhiqiang;Wu, Tonglei;Shi, Qiumei;Bai, Liya;Shi, Qiumei

作者机构：

关键词： Albiflorin; Enteritis; Inflammation; Oxidative stress;

NF-kappa B

; NLRP3

期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：5.714； 五年影响因子：5.596 ）

ISSN： 1567-5769

年卷期： 2022 年 109 卷

页码：

收录情况： SCI

摘要： Methotrexate (MTX) treats various diseases but also damages intestinal barrier and leads to enteritis. Albiflorin (ALB) has a variety of pharmacological effects, including antioxidant, anti-inflammation and anti-apoptosis. In the present study, we evaluated the therapeutic effect of ALB on MTX-induced enteritis and investigated the possible mechanisms involved. Male SD rats were intraperitoneally injected with 7 mg/kg MTX for three consecutive days to establish the enteritis model. ALB (20 or 40 mg/kg/day) was intragastrically administrated since two days prior MTX treatment and lasted for six days. We found that ALB treatment increased body weight and intestinal weight of rats with MTX injection. The disease activity index (DAI) score was also decreased after ALB administration. In histological examination, ALB treatment attenuated inflammatory cells infiltration and promoted survival of goblet cells. In detection of inflammatory-associated factors, ALB treatment decreased CD68(+) cells infiltration, inhibited myeloperoxidase activity, and suppressed intercellular cell adhesion molecule 1 and cyclooxygenase-2 expression. Additionally, ALB reduced malondialdehyde, glutathione levels, inhibited superoxide dismutase activity and suppressed reactive oxygen species production. Moreover, ALB treatment effectively inhibited NLRP3, as well as caspase 1 p20 and interleukin (IL)-113 and 18 expression. Finally, nuclear factor-kappa B (NF-kappa B) p65 phosphorylation and nuclear translocation were also demonstrated to be blocked upon ALB treatment. In conclusion, our findings indicated that ALB alleviated MTX-induced enteritis via inhibiting the NF-kappa B/NLRP3 pathway.

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