文献类型： 外文期刊

第一作者： Altimira, Fabiola

作者： Altimira, Fabiola;Arias-Aravena, Matias;Real, Nicolas;Correa, Pablo;Godoy, Sebastian;Vitta, Nancy;Tapia, Eduardo;Jian, Ling;Gonzalez, Carolina;Castro, Jean Franco;Zamora, Olga;Vergara, Cristina

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关键词： Beauveria pseudobassiana; entomopathogenic fungi (EPF); nonribosomal peptide synthetase (NRPS); polyketide synthase (PKS); extracellular enzymes; insecticidal activity

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

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年卷期： 2022 年 8 卷 3 期

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收录情况： SCI

摘要： The entomopathogenic fungus Beauveria pseudobassiana strain RGM 2184 can reach a maximum efficacy of 80% against the quarantine pest Lobesia botrana in field assays. In this study, the RGM 2184 genome was sequenced, and genome mining analyses were performed to predict the factors involved in its insecticidal activity. Additionally, the metabolic profiling of the RMG 2184 culture's supernatants was analyzed by mass spectrometry, and the insecticidal activity from one of these extracts was evaluated in Galleria mellonella larvae. The genome analysis resulted in 114 genes encoding for extracellular enzymes, four biosynthetic gene clusters reported as producers of insecticidal and bactericidal factors (oosporein, beauvericin, desmethylbassianin, and beauveriolide), 20 toxins, and at least 40 undescribed potential biocontrol factors (polyketides and nonribosomal peptides). Comparative genomic analysis revealed that 65-95% of these genes are Beauveria genus-specific. Metabolic profiling of supernatant extracts from RGM 2184 cultures exhibited secondary metabolites such as beauveriolide, oosporein, inflatin C, and bassiatin. However, a number of detected metabolites still remain undescribed. The metabolite extract caused 79% mortality of Galleria mellonella larvae at 28 days. The results of this research lay the groundwork for the study of new insecticidal molecules.

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