Alkaline phosphatase-triggered dual-signal immunoassay for colorimetric and electrochemical detection of zearalenone in cornmeal

文献类型： 外文期刊

第一作者： Shang, Cuiling

作者： Shang, Cuiling;Li, Yansong;Tang, Song;Ren, Honglin;Hu, Pan;Lu, Shiying;Zhou, Yu;Zhang, Qi;Tang, Xiaoqian;Li, Peiwu;Zhou, Yu

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关键词： Prussia blue nanoparticles; Alkaline phosphatase; Zearalenone; Dual-signal sensor

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：9.221； 五年影响因子：7.676 ）

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年卷期： 2022 年 358 卷

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收录情况： SCI

摘要： Zearalenone (ZEN), as one of the most important foods and feeds pollutant mycotoxins, is threatening human and animal health. Here, we have developed a dual-signal immunoassay triggered by alkaline phosphatase (ALP) for detecting ZEN in cornmeal. The immobilized ZEN-bovine serum albumin and free ZEN in the sample were competitively bound to an anti-ZEN monoclonal antibody (McAb). Then, the ALP labeled goat anti-mouse IgG (ALP-IgG) combined with the McAb. ALP catalyzed ascorbic acid 2-phosphate to produce L-ascorbic acid (AA). AA converted potassium ferricyanide (K-3[Fe(CN)(6)]) to potassium ferrocyanide (K-4[Fe(CN)(6)]), which reacted with ferric ion (III) to promote the formation of Prussian blue nanoparticles (PB NPs). Consequently, the solution appeared multicolor changes. Meanwhile, PB NPs had a maximum absorption peak at 700 nm. It could be monitored by an UV-vis spectrometer. As an electron transfer medium, K-3[Fe(CN)(6)] was consumed gradually along with PB NPs formation. Therefore, electrochemical detection was also used for detecting ZEN. Under the optimum conditions, the logarithm of ZEN concentration showed a good linear relationship with the absorbance from 0.2 to 0.8 ng/mL (R-2 = 0.987) and the differential pulse voltammetry peak current from 0.125 to 0.5 ng/mL (R-2 = 0.993). The limits of detection of colorimetric and electrochemical methods were 0.04 and 0.08 ng/mL, respectively. The recovery rates of ZEN from cornmeal samples were in the range of 80-120%, and relative standard deviations were lower than 10%. The results demonstrated that the dual-signal immunoassay has a high sensitivity for ZEN detection in corn samples.

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