Characterization of chicken p53 transcriptional function via parallel genome- wide chromatin occupancy and gene expression analysis

文献类型： 外文期刊

第一作者： Chen, Zhijie

作者： Chen, Zhijie;Cui, Lu;Xu, Li;Liu, Zheyi;Liang, Yumeng;Li, Xuefeng;Zhang, Yanhui;Liu, Shengwang;Li, Hai;Chen, Zhijie;Li, Yijing;Li, Hai

作者机构：

关键词： chicken; p53; chromatin immunoprecipitation sequencing; RNA sequencing

期刊名称：POULTRY SCIENCE （ 影响因子：4.014； 五年影响因子：4.192 ）

ISSN： 0032-5791

年卷期： 2022 年 101 卷 11 期

页码：

收录情况： SCI

摘要： The tumor suppressor p53, which acts primarily as a transcription factor, can regulate infec-tions from various viruses in chickens. However, the underlying mechanisms of the antiviral functions of chicken p53 (chp53) remain unclear due to the lack of detailed information on its transcriptional regulation. Here, to gain comprehensive insights into chp53 tran-scriptional regulatory function in a global and unbiased manner, we determined the genome-wide chromatin occupancy of chp53 by chromatin immunoprecipitation, which was followed by sequencing and chp53-mediated gene expression profile by RNA sequencing using chemi-cally immortalized leghorn male hepatoma (LMH) cells with ectopic expression of chp53 as the model. The integrated parallel genome-wide chromatin occupancy and gene expression analysis characterized chp53 chro-matin occupancy and identified 754 direct target genes of chp53. Furthermore, functional annotation and cross -species comparative biological analyses revealed the con-served key biological functions and DNA binding motifs of p53 between chickens and humans, which may be due to the consensus amino acid sequence and structure of p53 DNA-binding domains. The present study, to our knowledge, provides the first comprehensive characteri-zation of the chp53 transcriptional regulatory network, and can possibly help to improve our understanding of p53 transcriptional regulatory mechanisms and their antiviral functions in chickens.

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