Construction of pseudorabies virus variant attenuated vaccine: codon deoptimization of US3 and UL56 genes based on PRV gE/TK deletion strain

文献类型： 外文期刊

第一作者： Xu, Mengwei

作者： Xu, Mengwei;Zhu, Laixu;Liu, Yamei;Chen, Saisai;Wei, Ziwen;Zheng, Yating;Tong, Ling;Wang, Zhisheng;Wang, Jichun;Zhang, Chuanjian;Xu, Mengwei;Zhu, Laixu;Liu, Yamei;Chen, Saisai;Wei, Ziwen;Zheng, Yating;Tong, Ling;Wang, Zhisheng;Wang, Jichun;Zhang, Chuanjian;Xu, Mengwei;Zhu, Laixu;Liu, Yamei;Chen, Saisai;Wei, Ziwen;Zheng, Yating;Tong, Ling;Wang, Zhisheng;Wang, Jichun;Zhang, Chuanjian;Xu, Mengwei;Zhu, Laixu;Fei, Rongmei;Ge, Aimin

作者机构：

关键词： pseudorabies virus; attenuation; immunogenicity; codon deoptimization; US3-S; UL56

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Since 2011, pseudorabies based on the pseudorabies virus (PRV) variant has emerged as a serious health issue in pig farms in China. The PRV gE/TK or gE/gI/TK deletion strains protect against emerging PRV variants. However, these variants may cause lethal infections in newborn piglets without PRV antibodies. Previous studies have shown that codon deoptimization of a virulence gene causes virus attenuation. Accordingly, we deoptimized US3-S (US3 gene encoding a short isoform that represents approximately 95% of the total US3 transcription) and UL56 genes (first 10 or all codons) of PRV gE/TK deletion strain (PRV Delta TK&gE-AH02) to generate six recombinant PRVs through bacterial artificial chromosome technology. In swine testicular cells, recombinant PRVs with all codon deoptimization of US3-S or UL56 genes were grown to lower titers than the parental virus. Notably, US3-S or UL56 with all codon deoptimization reduced mRNA and protein expressions. Subsequently, the safety and immunogenicity of recombinant PRVs with codon deoptimization of US3-S or UL56 are evaluated as vaccine candidates in mice and piglets. The mice inoculated with recombinant PRVs with codon deoptimization of US3-S or UL56 showed exceptional survival ability without severe clinical signs. All codons deoptimized (US3-S and UL56) significantly decreased virus load and attenuated pathological changes in the brains of the mice. Moreover, the protection efficiency offered by recombinant PRVs with codon deoptimization of US3-S or UL56 showed similar effects to PRV Delta TK&gE-AH02. Remarkably, the 1-day-old PRV antibody-negative piglets inoculated with PRV Delta TK&gE-US3-ST-CD (a recombinant PRV with all codon deoptimization of US3-S) presented no abnormal clinical symptoms, including fever. The piglets inoculated with PRV Delta TK&gE-US3-ST-CD showed a high serum neutralization index against the PRV variant. In conclusion, these results suggest using codon deoptimization to generate innovative live attenuated PRV vaccine candidates.

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