Transcriptional and proteomic analysis

文献类型： 外文期刊

第一作者： Liu, Xiao-Jing

作者： Liu, Xiao-Jing;Bao, Xing;Wang, Meng-Yu;Li, Xiao-Man;Wang, Xu-Jing;Wang, Zhi-Xing;Wang, Xu-Jing;Wang, Zhi-Xing

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关键词： Gene edit; proteome; transcriptome; unintended effects

期刊名称：GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2164-5698

年卷期： 2023 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Unintended effects of gene edit crops may pose safety issues. Omics is a useful tool for researchers to evaluate these unexpected effects. Transcriptome and proteomics analyses were performed for two gene editors, CRISPR-Cas9 and adenine base editor (ABE) gene edit rice, as well as corresponding wild-type plants (Nipponbare). Transcriptome revealed 520 and 566 rice differentially expressed genes (DEGs) in the Cas9/Nip and ABE/Nip comparisons, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most DEGs participated in metabolism of terpenoids and polyketones, plant-pathogen interactions, and plant signal transduction. It mainly belongs to environmental adaptation. Proteomics revealed 298 and 54 rice differentially expressed proteins (DEPs) in the Cas9/Nip and ABE/Nip comparisons, respectively. KEGG pathway enrichment analysis showed that most DEPs participated in the biosynthesis of secondary metabolite and metabolic pathways. According to integrated transcriptomes and proteomics analysis, the results showed that no newly generated genes were identified as new transcripts of these differentially expressed genes, and gene edit tools had little effect on rice transcription levels and no new proteins were generated in the gene-edited rice.

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