The Q-interacted protein QIP3 recruits TaTPL to regulate spike architecture in wheat

文献类型： 外文期刊

第一作者： Yang, Ziyi

作者： Yang, Ziyi;Bai, Wanqing;Huang, Shuxian;Wang, Yufan;Zhang, Yunwei;Sun, Jiaqiang;Guo, Guanghui;Zhou, Yun

作者机构：

关键词： wheat; spike architecture; QIP3; EAR motif; TOPLESS

期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）

ISSN： 0960-7412

年卷期： 2025 年 122 卷 2 期

页码：

收录情况： SCI

摘要： Spike architecture is a critical determinant of grain yield in wheat; yet the regulatory mechanisms remain poorly understood. Here, we demonstrate that the AP2 transcription factor Q directly represses the expression of TaMYB30-6A, a gene associated with spike length in wheat. We further identify QIP3 as a Q-interacting protein harboring an N-terminal EAR motif. Simultaneously, we reveal that QIP3 exhibits transcriptional repression activity, dependent on the EAR motif, and physically interacts with the transcriptional corepressor TaTPL. Importantly, the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9)-generated qip3-aabbdd mutants exhibit reduced plant height and increased spike length phenotypes. Furthermore, RNA-seq and RT-qPCR assays show that QIP3 negatively regulates the expression of the Q target gene TaMYB30-6A in wheat. Collectively, we propose that the EAR motif-containing QIP3 interacts with Q to regulate spike architecture by recruiting the transcriptional corepressor TaTPL in wheat.

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