microRNA-8514-5p regulates adipokinetic hormone/corazonin-related peptide receptor to affect development and reproduction of Plutella xylostella

文献类型： 外文期刊

第一作者： Pang, Senbo

作者： Pang, Senbo;Wang, Shuo;Asad, Muhammad;Yu, Jiajing;Lin, Guifang;Sun, Cuiying;Huang, Pengrong;Chang, Yanpeng;Yang, Guang;Pang, Senbo;Wang, Shuo;Asad, Muhammad;Yu, Jiajing;Lin, Guifang;Sun, Cuiying;Huang, Pengrong;Chang, Yanpeng;Yang, Guang;Pang, Senbo;Wang, Shuo;Asad, Muhammad;Yu, Jiajing;Lin, Guifang;Sun, Cuiying;Huang, Pengrong;Chang, Yanpeng;Yang, Guang;Pang, Senbo;Wang, Shuo;Asad, Muhammad;Yu, Jiajing;Lin, Guifang;Sun, Cuiying;Huang, Pengrong;Chang, Yanpeng;Yang, Guang;Pang, Senbo;Wang, Shuo;Asad, Muhammad;Yu, Jiajing;Lin, Guifang;Sun, Cuiying;Huang, Pengrong;Chang, Yanpeng;Yang, Guang;Chen, Jinzhi;Wei, Hui

作者机构：

关键词： Dicer1; Plutella xylostella; miRNA; adipokinetic hormone/corazonin-related peptide receptor; development and reproduction

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUND: Dicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella, but the mechanism behind this phenomenon is not clear. This study aims to identify differentially-expressed genes and miRNAs in the Dicer1-knockout strain (Delta PxDcr-1) and assess their impact on the reproduction and development of P. xylostella. RESULTS: The knockout of Dicer1 affected the expression of genes including the adipokinetic hormone/corazonin-related peptide receptor (PxACPR). The expression of PxACPR was upregulated, and the expression of miR-8514-5p was downregulated in Delta PxDcr-1 of P. xylostella. The dual luciferase reporter assay and pull-down assay showed that miR-8514-5p bound to PxACPR in vitro and in vivo. The expression profiles demonstrated a negative correlation between PxACPR mRNA and miR-8514-5p in different developmental stages of the wild-type strain. Both the miR-8514-5p agomir and double-stranded RNA of ACPR (dsPxACPR) injected into the pre-pupae inhibited the mRNA level of PxACPR, causing high mortality and deformity of pupae, and low fecundity and hatching rate, which were consistent with the phenotype of Delta PxDcr-1. The injection of miR-8514-5p antagomir caused a similar phenotype to the injection of miR-8514-5p agomir. Additionally, the injection of miR-8514-5p antagomir significantly rescued the phenotype caused by dsPxACPR. CONCLUSION: These results indicate that miR-8514-5p affects the development and reproduction of P. xylostella by regulating PxACPR, and the homeostasis of PxACPR expression is essential for the development and reproduction of P. xylostella. (c) 2024 Society of Chemical Industry.

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