Integrative transcriptome and whole-genome bisulfite sequencing analyses of a temperature-sensitive albino tea plant cultivar
文献类型： 外文期刊
第一作者： Zhang, Kexin
作者： Zhang, Kexin;Feng, Xia;Liu, Ying;Yang, Yajun;Hao, Xinyuan;Wang, Xinchao;Wang, Lu;Hao, Xinyuan;Li, Dongliang;Wang, Xinchao;Wang, Lu
作者机构：
期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：6.4； 五年影响因子：5.9 ）
ISSN： 0031-9317
年卷期： 2023 年 175 卷 6 期
页码：
收录情况： SCI
摘要： Green tea made from albino buds and leaves has a strong umami taste and aroma. The cultivar 'Zhonghuang 2' (ZH2, Camellia sinensis) is a natural mutant with young shoots that are yellow in spring and green or yellow-green in summer. However, the mechanism of leaf color change remains unclear. Here, we found that young shoots of ZH2 were yellow at low temperature (LT) and green at high temperature (HT), indicating that ZH2 is a temperature-sensitive cultivar. Transmission electron microscopy analysis showed that the grana in the chloroplasts of young shoots grown at LT were poorly stacked, which caused a lack of photoreactions and chlorophyll. RNA-seq results showed 1279 genes differentially expressed in the young shoots grown at LT compared with those at HT, including genes related to cytochrome synthesis, chloroplast development, photosynthesis, and DNA methylation. A whole-genome bisulfite sequencing assay revealed that the dynamics of DNA methylation levels in the CG, CHG, and CHH contexts decreased under LT, and the change was most obvious in the CHH context. Furthermore, 72 genes showed significant changes in both expression and DNA methylation levels, and most of them were related to cytochrome synthesis, chloroplast development, photosynthesis, transcription factors, and signaling pathways. These results demonstrate that DNA methylation is involved in the LT-regulated albino processes of ZH2. Changes in DNA methylation levels were associated with changes in gene expression levels, affecting the structure and function of chloroplasts, which may have a phenotypic impact on shoot and leaf color.
分类号：
- 相关文献
作者其他论文 更多>>
-
Synergistic antimicrobial efficacy of glabrol and colistin through micelle-based co-delivery against multidrug-resistant bacterial pathogens
作者：Liu, Ying;Zhang, Mengying;Qu, Shaoqi;Liu, Ying;Mei, Chen;Wang, Hongjun;Cai, Yawei;Wu, Shuaicheng
关键词：Gram-negative bacteria; Colistin; Glabrol; Synergistic antibacterial; Micelle
-
Revealing the Molecular Regulatory Mechanism of Flavonoid Accumulation in Tender Leaves of Tea Plants by Transcriptomic and Metabolomic Analyses
作者：Shan, Ruiyang;You, Xiaomei;Kong, Xiangrui;Zhang, Yazhen;Li, Xinlei;Chen, Changsong;Zhang, Yongheng;Wang, Lu;Wang, Xinchao
关键词：
Camellia sinensis ; transcriptomics; flavonoids; regulatory network -
Interactions, properties and lipid digestibility of attractive Pickering emulgels formed by sequential addition of oppositely charged nanopolysaccharides
作者：Guo, Shasha;Ji, Xingxiang;Guo, Shasha;Li, Jun;Huang, Luyao;Guo, Shasha;Wan, Zhangmin;Niu, Xun;Xu, Junhua;Liu, Ying;Bai, Long;Lu, Yi;Rojas, Orlando J.;Jiao, Wenjuan;Zheng, Jianan;Li, Bin;Bai, Long;Lu, Yi;Rojas, Orlando J.;Rojas, Orlando J.
关键词：
-
Structural characterization and hypolipidemic activity of a hetero-galactan purified from Sanghuangporus vaninii based on modulation of TLR4/NF-κB pathway
作者：Hao, Jie;Zhu, Yanfeng;Li, Zhige;Wang, Lu;Qu, Yidi;Wang, Di;Zhang, Yongfeng;Li, Lanzhou;Wang, Di;Yu, Hailong;Qi, Liangliang
关键词：Sanghuangporus vaninii; Polysaccharide; Hypolipidemic efficacy; Inflammation; TLR4/NF-kappa B
-
Two leucine-rich repeat receptor-like kinases initiate herbivory defense responses in tea plants
作者：Jiang, Qi;Ding, Changqing;Feng, Lingjia;Wu, Zhenwei;Liu, Yujie;He, Lintong;Liu, Chuande;Wang, Lu;Zeng, Jianming;Huang, Jianyan;Ye, Meng
关键词：
-
CsCIPK20 Improves Tea Plant Cold Tolerance by Modulating Ascorbic Acid Synthesis Through Attenuation of CsCSN5-CsVTC1 Interaction
作者：Di, Taimei;Wu, Yedie;Wang, Jie;He, Mingming;Huang, Jianyan;Li, Nana;Hao, Xinyuan;Ding, Changqing;Zeng, Jianming;Yang, Yajun;Wang, Xinchao;Wang, Lu
关键词：ascorbic acid; CsCIPK20; CsVTC1; low temperature
-
Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum
作者：Zhi, Yan;Mei, Chen;Liu, Zhenyi;Liu, Ying;Wang, Hongjun
关键词：Glutathione reductase;
Av. paragallinarum ; Oxidative stress; Bacterial virulence; Redox homeostasis; Metabolic adaptation
