Bimetallic Nanozyme-Assisted Immunoassay for the Detection of Acetamiprid in Vegetables

文献类型： 外文期刊

第一作者： Liu, Beibei

作者： Liu, Beibei;Zhai, Rongqi;Liu, Guangyang;Huang, Xiaodong;Lv, Jun;Chen, Jing;Liu, Junjiang;Xu, Donghui;Chen, Ge;Liu, Beibei;Zhang, Jie;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Jin, Maojun;Wang, Jing

作者机构：

关键词： Indirect competitive immunoassay; Bimetallic Au@Pt nanozyme; Acetamiprid; Residue; Vegetables

期刊名称：ACS APPLIED NANO MATERIALS （ 影响因子：5.5； 五年影响因子：5.6 ）

ISSN：

年卷期： 2024 年 7 卷 18 期

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收录情况： SCI

摘要： The ability of conventional enzyme-linked immunosorbent assays to satisfy the increasing demand for advanced immunoassays is limited by their reliance on natural enzymes, low sensitivity, and narrow detection range. The development of nanozyme-assisted immunoassays is a viable approach to overcoming these constraints. Here, we report a one-step bimetallic nanozyme-assisted indirect competitive immunoassay to detect the pesticide acetamiprid in vegetables. Litchi-like bimetallic gold and platinum (Au@Pt) nanozymes with high catalytic properties and excellent biocompatibility were synthesized for this assay. The Au@Pt nanozymes were simple and inexpensive to prepare, exhibited stability and adjustable catalytic activity, and were directly conjugated with antibodies as signal probes, without the use of horseradish peroxidase-conjugated Affinipure goat antimouse IgG (IgG-HRP). Acetamiprid was shown to compete with the antigen to bind the Au@Pt probes. Subsequently, Au@Pt probes, which exhibited peroxidase-like activity, were used to catalyze the oxidation of colorless 3,3 ',5,5 '-tetramethylbenzidine, demonstrating the easy detection of acetamiprid through colorimetric signal monitoring. The linear range, sensitivity, relative standard deviation range, and limit of detection of the immunoassay were 1.85-327.19 mu g/L, 25.58 mu g/L, 0.46-10.35%, and 0.78 mu g/L, respectively, satisfying the detection requirements. In conclusion, the method exhibited high sensitivity, reproducibility, and a wide linear range for the detection of acetamiprid, while eliminating the need for IgG and simplifying the experimental procedure. This new indirect competitive immunoassay has great potential value for on-site sensitive detection of acetamiprid in vegetables.

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