Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

文献类型： 外文期刊

第一作者： Wang, Zhi-Peng

作者： Wang, Zhi-Peng;Zhang, Xin-Yue;Ma, Yan;Ye, Jing-Run;Chen, Wei;Jiang, Jing;Wang, Hai-Ying

作者机构： Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China;Suzhou Univ Sci & Technol, Sch Environm Sci & Engn, Suzhou 215009, Jiangsu, Peoples R China;Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Sustainable Dev Polar Fishery, Qingdao 266071, Peoples R China

关键词： Lipid; Soy molasses; Whey powder; alpha-Galactosidase; beta-Galactosidase

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 2020影响因子：6.04； 五年影响因子：6.485 ）

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年卷期： 2021 年 14 卷 1 期

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收录情况： SCI

摘要： Background: Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. Results: In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C-16:0, C-18:0, C-18:1, and C-18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three alpha-galactosidases and five beta-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key alpha-galactosidases, and GalG was key beta-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. beta-galactosidase was responsible for lactose hydrolysis; sucrase and alpha-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. Conclusions: This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess. Graphic abstract

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