Colloidal gold and fluorescent immunochromatographic test strips for canine parvovirus detection

文献类型： 外文期刊

第一作者： Zhang, Cheng-Qi

作者： Zhang, Cheng-Qi;Wan, Ying;Shi, Zheng-Wang;Luo, Jun-Cong;Tian, Hong;Zheng, Hai-Xue;Zhang, Cheng-Qi;Li, Hong-Ye;Li, Shuang-Shuang;Li, Yun-Zhen;Dai, Xin-Yu;Bai, Xue

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关键词： Canine parvovirus; Colloidal gold strip; Antigen test; Fluorescent immunochromatographic

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.0； 五年影响因子：5.2 ）

ISSN： 0175-7598

年卷期： 2023 年 107 卷 15 期

页码：

收录情况： SCI

摘要： Canine parvovirus (CPV) is an acute and highly infectious virus causing disease in puppies and, thus, affecting the global dog industry. The current CPV detection methods are limited by their sensitivity and specificity. Hence, the current study sought to develop a rapid, sensitive, simple, and accurate immunochromatographic (ICS) test to detect and control the spread and prevalence of CPV infection. More specifically, 6A8, a monoclonal antibody (mAb) with high specificity and sensitivity, was obtained by preliminary screening. The 6A8 antibody was labelled with colloidal gold particles. Subsequently, 6A8 and goat anti-mouse antibodies were coated onto a nitrocellulose membrane (NC) as the test and control lines, respectively. Furthermore, 6A8 and rabbit IgG antibodies were labelled with fluorescent microspheres and evenly sprayed onto a glass fibre membrane. Both strips could be prepared in 15 min with no noticeable cross-reactivity with other common canine intestinal pathogens. The strips were simultaneously used to detect CPV in 60 clinical samples using real-time quantitative PCR, hemagglutination, and hemagglutination inhibition assays. The colloidal gold (fluorescent) ICS test strip was stable for 6 (7) and 4 (5) months at 4 degrees C and room temperature (18-25 degrees C). Both test strips were easy to prepare and rapidly detected CPV with high sensitivity and specificity. Moreover, the results were easily interpretable. This study establishes a simple method for two CPV diseases, colloidal gold and fluorescent immunochromatographic (ICS) test strips.

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