Two P450 genes, CYP6SN3 and CYP306A1, involved in the growth and development of Chilo suppressalis and the lethal effect caused by vetiver grass

文献类型： 外文期刊

第一作者： Lu, Yanhui

作者： Lu, Yanhui;Bai, Qi;Li, Qiang;Zheng, Xusong;Tian, Junce;Guo, Jiawen;Xu, Hongxing;Lu, Zhongxian

作者机构：

关键词： Cytochrome P450 monooxygenase; Lethality; Vetiver grass

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2022 年 223 卷

页码：

收录情况： SCI

摘要： Chilo suppressalis is a widely distributed pest occurring in nearly all paddy fields, which has developed high level resistance to different classes of insecticides. Vetiver grass has been identified as a dead-end trap plant for the alternative control of C. suppressalis. In this study, two cytochrome P450 monooxygenase (P450) genes, CsCYP6SN3 and CsCYP306A1, were identified and characterized, which are expressed at all developmental stages, with the highest expression in the midguts and fat bodies of 3rd instar larvae. Vetiver significantly inhibited the expression levels of CsCYP6SN3 and CsCYP306A1 in 3rd larvae after feeding. RNA interference showed that silencing CsCYP6SN3 and CsCYP306A1 genes dramatically reduced the pupation rate and pupa weight. Feeding on vetiver after silencing CsCYP6SN3 and CsCYP306A1 led to higher mortality compared with feeding on rice. In conclusion, these findings indicated that the expression levels of CsCYP6SN3 and CsCYP306A1 were associated with the lethal effect of vetiver against C. suppressalis larvae and functional knowledge about these two detoxification genes could provide new targets for agricultural pest control.

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