Development of two sets of tobacco chromosome segment substitution lines and QTL mapping for agronomic and disease resistance traits

文献类型： 外文期刊

第一作者： Si, Huan

作者： Si, Huan;Zan, Yanjun;Song, Shiyang;Wang, Yongda;Jiang, Caihong;Pan, Xuhao;Xiao, Zhiliang;Wen, Liuying;Sun, Yiwen;Liu, Dan;Cheng, Lirui;Yang, Aiguo;Wang, Dong;Liu, Wanfeng;Pu, Wenxuan;Li, Xiaoxu;Mao, Hui;Yang, Xingyou

作者机构：

关键词： Tobacco; CSSLs; QTL mapping; Agronomic traits; Disease resistance

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 226 卷

页码：

收录情况： SCI

摘要： Chromosome segment substitution lines (CSSLs) represent a powerful genetic resource for quantitative trait loci (QTL) mapping, gene cloning and breeding. Here, we developed two sets of CSSLs consisting of 245 and 128 unique lines, which derived from OX2028 x K326 and Samsun x K326 crosses. On average, each CSSL carried 1.8 and 2.9 introgressed segments in the two sets, with an average physical segment length of approximately 34.3 Mb and 27.6 Mb, respectively. These CSSLs covered similar to 97 % and similar to 77 % of the genomes of OX2028 and Samsun, respectively. By performing QTL mapping based on best linear unbiased prediction (BLUP) of phenotypic traits, we identified a total of 64 QTLs associated with six agronomic traits and three disease resistance traits. These QTLs explained phenotypic variation ranging from 1.5 % to 50.8 %. Among them, 22 QTLs detected in OX2028 derived population and 42 detected in Samsun derived CSSLs. Notably, a new QTL for tobacco leaf width, qLW1-1 was narrowed down to an 8-Mb interval on chromosome 1, and NtZY01G00114, encoding an auxin-response factor protein, was considered as the candidate gene. Our study provides valuable genetic resources for tobacco breeding and enhances our understanding of the genetic basis of complex traits in tobacco.

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