Production of aromatic three-line hybrid rice using novel alleles of BADH2

文献类型： 外文期刊

第一作者： Hui, Suozhen

作者： Hui, Suozhen;Li, Huijuan;Mawia, Amos Musyoki;Zhou, Liang;Ahmad, Shakeel;Lai, Changkai;Wang, Jingxin;Jiao, Guiai;Xie, Lihong;Shao, Gaoneng;Sheng, Zhonghua;Tang, Shaoqing;Wei, Xiangjin;Hu, Shikai;Hu, Peisong;Cai, Jinyang;Wang, Jianlong

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关键词： grain aroma improvement; 2-AP; gene editing; GABA; three-line indica hybrid rice

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

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收录情况： SCI

摘要： Aroma is a key grain quality trait that directly influences the market price of rice globally. Loss of function of betaine aldehyde dehydrogenase 2 (OsBADH2) affects the biosynthesis of 2-acetyl-1-pyrroline (2-AP), which is responsible for aroma in fragrant rice. The current study was aimed at creating new alleles of BADH2 using CRISPR/Cas9 gene editing technology under the genetic background of the japonica Ningjing 1 (NJ1) and indica Huang Huazhan (HHZ) varieties. Sensory evaluation and analysis using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) showed that the grains of the four homozygous T-1 lines with new alleles of BADH2 (nj1-cr (BADH2)-1, nj1-cr (BADH2)-2, hhz-cr (BADH2)-1 and hhz-cr (BADH2)-2) produced moderate fragrance and had significantly increased 2-AP content compared with wild-types. Moreover, there were no significant differences in the amylose content and gelatinization temperature among the four lines with new alleles of BADH2 to the wild-types. Thereafter, we crossed the HHZ background new alleles of BADH2 with CMS line Taonong 1A (TN1A) to produce a three-line hybrid variety B-Tao-You-Xiangzhan (BTYXZ) with increased grain aroma. The 2-AP content in grains of the improved BTYXZ-1 and BTYXZ-2 reached at 26.16 and 18.74 mu g/kg, and the gel consistency of BTYXZ-1 and BTYXZ-2 increased significantly by 9.1% and 6.5%, respectively, compared with the wild-type Tao-You-Xiangzhan (TYXZ). However, the gamma-aminobutyric acid (GABA) content in the improved three-line hybrid rice BTYXZ-1 (5.6 mg/100 g) and BTYXZ-2 (10.7 mg/100 g) was significantly lower than that of the TYXZ. These results demonstrated that CRISPR/Cas9 gene editing technology could be successfully utilized in improving aroma in non-fragrant japonica and indica varieties. In addition, the newly developed BADH2 alleles provided important genetic resources for grain aroma improvement in three-line hybrid rice.

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