Obtaining a Monoclonal Antibody against a Novel Prometryn-Like Hapten and Characterization of Its Selectivity for Triazine Herbicides

文献类型： 外文期刊

第一作者： Xu, Lingyuan

作者： Xu, Lingyuan;Zhao, Jing;Lei, Xingmei;Zhao, Yun;Cui, Xueyan;She, Yongxin;Jin, Fen;Wang, Jing;Jin, Maojun;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Zhang, Xiuyuan;Hammock, Bruce D. D.;Hammock, Bruce D. D.

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关键词： prometryn; hapten; monoclonal antibody; immunoassays

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）

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年卷期： 2023 年 13 卷 1 期

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收录情况： SCI

摘要： In this study, a previously unreported 3-((4-(isopropylamino)-6-(methylthio)-1,3,5-triazin-2-yl) amino) butyric acid hapten was designed and synthesized. This maximized the exposure of the antigen-determinant isopropyl of prometryn to the immune system in animals to induce the production of anticipated highly specific anti-prometryn antibodies. The hapten has a molecular weight of 285.37 Da. The compound was confirmed by nuclear magnetic resonance hydrogen spectroscopy (H-1 NMR), nuclear magnetic resonance carbon spectroscopy (C-13 NMR), and high-resolution mass spectrometry (HRMS). By using the active ester approach, immunogens and coated antigens were created. Bovine serum albumin (BSA) was used as an immunogen, along with the successfully produced hapten, to immunize mice. The IC50 value of mouse monoclonal anti-prometryn antibody (mAb) 7D4 (the quantity of analyte that generated 50% prometryn inhibition) was 3.9 ng/mL. The anti-prometryn mAb was of the IgG1 subclass. The IC20 (80% binding level (B/B-0) of prometryn)-IC80 (20% binding level (B/B-0) of prometryn) range of the anti-prometryn monoclonal antibody standard curve working range was 0.9-18.1 ng/mL. The prepared mAb has good characteristics because it can specifically recognize prometryn, and the cross-reaction rates for ametryn, desmetryn, and terbumeton were 34.77%, 18.09%, and 7.64%, respectively. The cross-reaction rate with the other seven triazines was less than 1%. The hapten structure proposed can serve as an additional tool for modulating selectivity in detecting triazines.

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