RNA interference-mediated silencing of ctl13 inhibits innate immunity and development in stored pest Tribolium castaneum

文献类型： 外文期刊

第一作者： Ning, Mingxiao

作者： Ning, Mingxiao;Li, Qing;Fan, Lixia;Guo, Changying;Zhang, Bingchun;Ren, Xianfeng;Li, Jia;Li, Bin;Zhu, Jingxuan

作者机构：

关键词： RNAi; C -type lectins; Tribolium castaneum; Innate immune; Development

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2024 年 204 卷

页码：

收录情况： SCI

摘要： C-type lectins (CTLs) play a pivotal role in the regulation of insect immunity and growth, making them potential molecular targets for RNA interference (RNAi)-mediated pest control. Although multiple CTLs have been identified in the genomes of various insects, their specific functions and underlying molecular mechanisms remain unclear. In the present study, a novel CTL, Tcctl13 with a single CRD, was identified in Tribolium castaneum. . Tcctl13 is expressed in diverse immune-related tissues and developmental stages, with a notable increase in its expression upon exposure to lipopolysaccharides (LPS) and peptidoglycan (PGN). Molecular docking and enzyme-linked immunosorbent assay (ELISA) analyses revealed that TcCTL13 possesses the ability interacted with LPS and PGN. The binding and agglutinating activities of recombinant TcCTL13 (rTcCTL13) were demonstrated against both gram-negative and positive bacteria. After using RNAi to silence Tcctl13, , the expression of the eight antimicrobial peptide (AMP) genes was significantly reduced. In addition, knocking down Tcctl13 during the early larval or pupal stage hindered, the normal metamorphosis process in T. castaneum, , ultimately leading to the demise of all beetles. Further research showed that Tcctl13 and nine AMPs were significantly downregulation after 20-Hydroxyecdysone (20E) injection. Instead, the up-regulation of Tcctl13 and six AMPs was observed following interference with the 20E receptor (ecdysone receptor, EcR), indicating that the function of Tcctl13 is regulated by 20E in T. castaneum. . Collectively, these findings suggest that Tcctl13 plays a role in the regulation of innate immunity and development in T. castaneum, , offering a promising molecular target for managing insect pests using RNAi-based approaches.

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