Effects of Different Levels of Antarctic Krill Oil on the Ovarian Development of Macrobrachium rosenbergii

文献类型： 外文期刊

第一作者： Zheng, Xiaochuan

作者： Zheng, Xiaochuan;Yang, Jie;Liu, Xin;Sun, Cunxin;Zhou, Qunlan;Liu, Bo;Zheng, Xiaochuan;Yang, Jie;Liu, Xin;Sun, Cunxin;Zhou, Qunlan;Liu, Bo;Wang, Aimin;Chen, Jianming

作者机构：

关键词： functional feed; ovarian histology; giant freshwater prawn; reproductive hormones; vitellogenesis

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 22 期

页码：

收录情况： SCI

摘要： The lack of in-depth research and development on specialized functional feedfor broodstock gonad maturation represents a significant obstacle to the industrialization ofMacro-brachium rosenbergiibreeding. The potential of Antarctic krill oil to promote ovarian development incrustaceans has been established; however, the optimal application dose and potential regulatorymechanism inM. rosenbergiiremain unclear. This study established a gradient addition of Antarctickrill oil (0%, 1.5%, 3%, 4.5%, and 6%) for a period of eight weeks. Antarctic krill oil supplementationin the range of 3-4.5% resulted in a significant promotion of ovarian development, vitellogenesis,and reproductive hormone synthesis. The retinol metabolic signaling pathway, methyl farnesoate(MF) signaling pathway, steroid hormone signaling pathway, and ecdysone signaling pathway alongwith their specific molecules were found to be involved in the regulation of ovarian development ofM. rosenbergiiwith the use of Antarctic krill oil. The results demonstrate that the 4.5% supplementa-tion Antarctic krill oil in the diet is optimal for stimulating the secretion of reproductive hormones infemaleM. rosenbergii, thereby promoting vitellogenesis and ovarian development. Antarctic krill oil has been proven to be able to promote the ovarian development of crustaceans, but its optimal application dose and potential regulatory mechanism in Macrobrachium rosenbergii are still unclear. In this study, five isonitrogenous and isolipidic diets with gradient additions of Antarctic krill oil (0%, 1.5%, 3%, 4.5%, and 6%) were served exposed to 8 weeks of feeding. The results show that 3-4.5% Antarctic krill oil supplementation significantly increases the weight gain rate and specific growth rate of M. rosenbergii (p < 0.05). In addition, 3-4.5% Antarctic krill oil supplementation significantly increased the content of hemolymph vitellogenin (VTG) and the levels of reproductive hormones, including methyl farnesoate (MF), estradiol (E2), and progesterone (P4) (p < 0.05). The differences in ovarian index, oocyte volume, yolk granule deposition in oocytes, and the transcription levels of VTG genes in hepatopancreas and ovarian tissues demonstrated that the addition of Antarctic krill oil significantly promoted ovarian development and vitellogenesis, especially at the 4.5% addition level. In terms of molecular signaling, this study confirms that the retinol metabolic signaling pathway, MF signaling pathway, steroid hormone signaling pathway, and ecdysone signaling pathway, along with their specific molecules, such as Farnesoic acid-O-methyltransferase (FAMeT), retinoid x receptor (RXR), ecdysone receptor (EcR), and estrogen-related receptor (ERR), are involved in the regulation of the ovarian development of M. rosenbergii by adding Antarctic krill oil at appropriate doses. The findings indicate that the supplementation of 4.5% Antarctic krill oil in the diet is optimal for stimulating the secretion of reproductive hormones in female M. rosenbergii, thereby promoting vitellogenesis and ovarian development.

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