Selective increase of antibiotic-resistant denitrifiers drives N2O production in ciprofloxacin-contaminated soils

文献类型： 外文期刊

第一作者： Fang, Linfa

作者： Fang, Linfa;Deng, Yue;Lakshmanan, Prakash;Liu, Weibing;Zou, Wenxin;Zhang, Tong;Wang, Xiaozhong;Xiao, Ran;Chen, Xinping;Su, Xiaoxuan;Lakshmanan, Prakash;Lakshmanan, Prakash;Lakshmanan, Prakash;Tang, Xiufeng;Zhang, Jinbo;Zhang, Jinbo;Chen, Xinping

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关键词： N2O emission; Manure application; 15 N-labelling; Microbial community; Soil antibiotic residues

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2024 年 479 卷

页码：

收录情况： SCI

摘要： Agricultural systems significantly contribute to global N2O emissions, which is intensified by excessive fertilization and antibiotic residues, attracting global concerns. However, the dynamics and pathways of antibioticsinduced soil N2O production coupled with microbial metabolism remain controversial. Here, we explored the pathways of N2O production in agricultural soils exposed to ciprofloxacin (CIP), and revealed the underlying mechanisms of CIP degradation and the associated microbial metabolisms using 15N-isotope labeling and molecular techniques. CIP exposure significantly increases the total soil N2O production rate. This is attributed to an unexpected shift from heterotrophic and autotrophic nitrification to denitrification and an increased abundance of denitrifiers Methylobacillus members under CIP exposure. The most striking strain M. flagellatus KT is further discovered to harbor N2O-producing genes but lacks a N2O-reducing gene, thereby stimulating denitrification-based N2O production. Moreover, this denitrifying strain is probably capable of utilizing the byproducts of CIP as carbon sources, evidenced by genes associated with CIP resistance and degradation. Molecular docking further shows that CIP is well ordered in the catalytic active site of CotA laccase, thus affirming the potential for this strain to degrade CIP. These findings advance the mechanistic insights into N2O production within terrestrial ecosystems coupled with the organic contaminants degradation.

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