Development of homozygous transgenic Atropa belladonna plants with glyphosate resistance and high-yield scopolamine using metabolic engineering

文献类型： 外文期刊

第一作者： Zhang, Qiaozhuo

作者： Zhang, Qiaozhuo;Liang, Mengjiao;Liu, Yuanyuan;Yang, Chunxian;Zeng, Junlan;Liao, Zhihua;Qin, Jianbo;Liao, Zhihua;Lan, Xiaozhong;Lin, Min;Chen, Min;Wang, Jin

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关键词： Atropa belladonna; Homozygous lines; Glyphosate resistance; Scopolamine; Metabolic engineering

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）

ISSN： 0926-6690

年卷期： 2021 年 171 卷

页码：

收录情况： SCI

摘要： Atropa belladonna, one of the most important medicinal plants in China, is used to produce anticholinergic tropane alkaloids. Weeds severely reduce the agricultural productivity of A. belladonna in fields, and the low content of high-value scopolamine in planta limits its economic value. In this study, homozygous lines of A. belladonna, with glyphosate resistance and high yield of scopolamine, were developed using metabolic engineering technology for the first time. Two homozygous transgenic lines (T2GH03 and T2GH06), harboring G2EPSPS and HnH6H, were established and tested in the field. Glyphosate resistance assays demonstrated that the two homozygous transgenic lines could resist commercial recommended dose of glyphosate. Alkaloid analysis showed that the transgenic lines produced more scopolamine than wild-type plants. T2GH03 and T2GH06 produced scopolamine at the levels of 5.45 mg/g dry weight (DW) and 7.04 mg/g DW respectively in leaves, and 1.06 mg/g DW to 1.07 mg/g DW respectively in roots. Compared with wild-type plants, the scopolamine contents of homozygous transgenic lines increased by over 10 folds in leaves and nearly 5 folds in roots. To sum up, Atropa belladonna's new varieties with glyphosate resistance and high-yield scopolamine are developed, which are highly valuable for industrial production of scopolamine.

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