Screening, identification, metabolic pathway of di-n-butyl phthalate degrading Priestia megaterium P-7 isolated from long-term film mulched cotton field soil in Xinjiang

文献类型： 外文期刊

第一作者： Yi, Yuanyang

作者： Yi, Yuanyang;Zhang, Wei;Zhang, Zhidong;Wang, Yuxian;Liu, Wanqin;Zhang, Zhidong;Zhu, Jing;Gu, Meiying;Jia, Qiong;Zhang, Zhidong;Li, Xue;Mutalifu, Munire;Zhang, Zhidong;Jiang, Ling

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关键词： Di-n-butyl phthalate; biodegradation; whole-genome sequencing; metabolomics analysis; Priestia megaterium P-7

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

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年卷期： 2025 年 16 卷

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收录情况： SCI

摘要： Introduction Di-n-butyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) and is considered an emerging global pollutant. It may pose a significant threat to ecosystem and human health due to its residual hazards and accumulation in the environment. Bacteria-driven PAE biodegradation is considered an economical and effective strategy for remediating such polluted environments.Methods A DBP-degrading bacterium (P-7), was isolated from long-term film mulched cotton field soil. Its identity was confirmed via physiological, biochemical, and 16S rRNA gene analyses. The degradation conditions were optimized through single-factor experiments and response surface methodology (RSM).Furthermore, the whole-genome sequencing coupled with metabolomics was employed to elucidate metabolic mechanisms.Results Priestia megaterium P-7 (P. megaterium P-7) achieved 100% DBP removal within 20 h under optimal conditions and exhibited broad substrate specificity for other PAEs. Genomic analysis identified key genes (lip, aes, ybfF, estA, and yvaK) encoding esterases/hydrolases that initiate DBP catabolism, converting it to phthalic acid (PA). Subsequent decarboxylation (pdc, bsdCD, mdcACDH, and lysA) and dioxygenase-mediated steps integrated PA into the TCA cycle. Metabolomics revealed three degradation pathways: decarboxylation (DBP -> MBP -> BB -> BA -> Catechol), hydrolysis (DBP -> MBP -> PA -> PCA -> Catechol) and direct beta-oxidation (DBP -> DEP -> MEP -> PA -> Catechol).Conclusion P. megaterium P-7 demonstrates exceptional degradation efficiency, substrate versatility, and environmental stress tolerance, making it a promising candidate for bioremediation of organic pollutants in contaminated soil.

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