Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac

文献类型： 外文期刊

第一作者： Zhang, Yongjing

作者： Zhang, Yongjing;Zhang, Aijing;Li, Mengyuan;Guo, Shuyuan;He, Kanglai

作者机构：

关键词： Bacillus thuringiensis; microcapsule; nanoparticles; UV resistance; insecticidal activity

期刊名称：JOURNAL OF MICROENCAPSULATION （ 影响因子：3.142； 五年影响因子：2.933 ）

ISSN： 0265-2048

年卷期： 2021 年 38 卷 7-8 期

页码：

收录情况： SCI

摘要： Aim To prepare several novel microcapsules using chitosan (Cs) and Alginate (Alg) as coating materials, and nano-ZnO, nano-SiO2, nano-TiO2 as UV protective agents for improving UV resistance of Cry1Ac. Methods Microcapsules were prepared by the layer-by-layer (LbL) self-assembly technique and electrostatic adsorption. The morphologies were observed by scanning electron microscopy (SEM), and the stability under UV radiation was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and bioassay. Results SEM showed that nano-ZnO and nano-TiO2 could be adsorbed on the negatively charged MC with the outermost layer being Alg, while nano-SiO2 could be adsorbed on the positively charged MC with Cs as the outermost layer. SDS-PAGE and bioassay showed that nano-ZnO and nano-SiO2 could provide effective UV protection after 8 h UV irradiation (p > 0.05), and nano-TiO2 could provide effective UV protection after 4 h UV irradiation (p > 0.05). Conclusion The microcapsules loaded with nanoparticles provided excellent UV resistance for Cry1Ac.

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