Immune responses induced by a recombinant C-strain of classical swine fever virus expressing the F317L protein of African swine fever virus

文献类型： 外文期刊

第一作者： Li, Shuwen

作者： Li, Shuwen;Gao, Yuxuan;Zhai, Huanjie;Guan, Xiangyu;Yang, Xiaoke;Hou, Qinghe;Zhang, Xinyu;Li, Lian-Feng;Qiu, Hua-Ji;Li, Yongfeng;Li, Shuwen;Zhang, Xinyu;Huang, Shujian;Qiu, Hua-Ji;Wang, Xiao

作者机构：

关键词： African swine fever virus; F317L protein; Antigenicity; Cell-mediated immune responses

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2024 年 298 卷

页码：

收录情况： SCI

摘要： African swine fever (ASF), a highly infectious and devastating disease affecting both domestic pigs and wild boars, owes its etiology to African swine fever virus (ASFV). ASFV encodes more than 165 proteins. However, novel immunogenic proteins remain unknown. This study aimed to determine the antigenicity of the F317L protein (pF317L) of ASFV. The results revealed that pF317L was able to react with convalescent pig sera, indicating that pF317L could be a candidate antigen. The antigenic potential of pF317L expressed by rHCLV-F317L, a recombinant virus in the backbone of C-strain (a lapinized live attenuated classical swine fever virus) was further investigated in rabbits and pigs. The results revealed that antibodies and cell-mediated immune responses against pF317L were induced in either rabbits or pigs inoculated with rHCLV-F317L. Importantly, anti-pF317L antibodies from rabbits or pigs immunized with rHCLV-F317L significantly inhibited ASFV replication in vitro. In conclusion, pF317L demonstrates favorable immunogenic properties, positioning it as a promising candidate for the development of protective antigens in the ongoing endeavor to formulate efficacious ASF vaccine strategies.

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