Sporulation of several biocontrol fungi as affected by carbon and nitrogen sources in a two-stage cultivation system

文献类型： 外文期刊

第一作者： Liu, Xingzhong

作者： Liu, Xingzhong;Gao, Li

作者机构：

关键词： M. anisopliae;nutrition;P. lilacinus;sporulation

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

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收录情况： SCI

摘要： The development of fungal biopesticides requires the efficient production of large numbers spores or other propagules. The current study used published information concerning carbon concentrations and C:N ratios to evaluate the effects of carbon and nitrogen sources on sporulation of Paecilomyces lilacinus (IPC-P and M-14) and Metarhizium anisopliae (SQZ-1-21 and RS-4-1) in a two-stage cultivation system. For P. lilacinus IPCP, the optimal sporulation medium contained urea as the nitrogen source, dextrin as the carbon source at 1 g/L, a C:N ratio of 5:1, with ZnSO_4·7H_2O at 10 mg/L and CaCl_2 at 3 g/L. The optimal sporulation medium for P. lilacinus M-14 contained soy peptone as the nitrogen source and maltose as the carbon source at 2 g/L, a C:N ratio of 10:1, with ZnSO_4·7H_2O at 250 mg/L, CuSO_4·5H_2O at 10 mg/L, H_3BO_4 at 5 mg/L, and Na_2MoO_4·2H_2O at 5 mg/L. The optimum sporulation medium for M. anisopliae SQZ-1-21 contained urea as the nitrogen source, sucrose as the carbon source at 16 g/ L, a C:N ratio of 80:1, with ZnSO_4·7H_2O at 50 mg/L, CuSO_4·5H_2O at 50 mg/L, H_3BO_4 at 5 mg/L, and MnSO_4·H_2O at 10 mg/L. The optimum sporulation medium for M. anisopliae RS-4-1 contained soy peptone as the nitrogen source, sucrose as the carbon source at 4 g/L, a C:N ratio of 5:1, with ZnSO_4·7H_2O at 50 mg/L and H_3BO_4 at 50 mg/L. All sporulation media contained 17 g/L agar. While these results were empirically derived, they provide a first step toward low-cost mass production of these biocontrol agents.

分类号： Q93

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