Ac/Ds Transposition Activity in Transgenic Rice Population and DNA Flanking Sequence of Ds Insertion Sites

文献类型： 外文期刊

第一作者： Zhu, ZG

作者： Zhu, ZG;Fu, YP;Xiao, H;Hu, GC;Si, HM;Yu, YH;Sun, ZH

作者机构：

关键词： transposon Ac/Ds;transposition activity;flanking sequence;oryza sativa

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Agrobacterium mediated transgenic rice ( Oryza sativa L.) population with inserts of maize transposon Activator/Dissociation (Ac/Ds) was investigated. DNA sequences flanking the T-DNA were analyzed with inverse PCR. Results showed that 65.4% of the T-DNA was integrated in different locations of rice genome, and some T-DNA flanking sequences were located on certain chromosomes. A number of T-DNA was found to have inserted into protein coding regions. In order to induce transposition of the inserted Ds elements, 354 crosses of Ac x Ds and Ds x Ac were constructed. The excision frequency of Ds element trans-activated by Ac transposase was 22.7% in the F2 populations, and the transposition was confirmed with analyses of DNA sequences flanking the Ds elements, In addition to the transposition due to "cut-paste" mechanism, Ds can replicate itself and integrate into a new locus, and inaccurate excisions were also found. A proportion of DNA segments flanking the Ds elements showed no homologies to sequences published in GenBank, of which two were registered under the accession numbers AF355153 and AF355770. The strategy of using transposon tagging for rice genomics study was discussed.

分类号： Q94

  • 相关文献

[1]Characterization of autonomous Dart1 transposons belonging to the hAT superfamily in rice. Shimatani, Zenpei,Takagi, Kyoko,Eun, Chang-Ho,Hoshino, Atsushi,Terada, Rie,Johzuka-Hisatomi, Yasuyo,Iida, Shigeru,Tsugane, Kazuo,Shimatani, Zenpei,Hoshino, Atsushi,Terada, Rie,Iida, Shigeru,Tsugane, Kazuo,Maekawa, Masahiko,Takahara, Hiroyuki,Qian, Qian,Qian, Qian. 2009

[2]Event-specific qualitative and quantitative detection of transgenic rice Kefeng-8 by characterization of the transgene flanking sequence. Wang, Wei-xia,Lai, Feng-xiang,Fu, Qiang,Zhu, Ting-heng. 2012

[3]High-throughput generation of an activation-tagged mutant library for functional genomic analyses in tobacco. Liu, Feng,Zhang, Qian,Zhang, Zhiguo,Wu, Jinxia,Gong, Daping,Wang, Dawei,Cui, Mengmeng,Liu, Guanshan,Wang, Yuanying.

[4]Activation tagging, an efficient tool for functional analysis of the rice genome. Wan, Shuyan,Wu, Jinxia,Zhang, Zhiguo,Sun, Xuehui,Lv, Yaci,Gao, Ci,Ning, Yingda,Ma, Jun,Guo, Yupeng,Zhang, Qian,Zheng, Xia,Lu, Tiegang,Lv, Yaci,Gao, Ci,Ning, Yingda,Zhang, Caiying,Ma, Zhiying.

[5]Identification of Genomic Insertion and Flanking Sequence of G2-EPSPS and GAT Transgenes in Soybean Using Whole Genome Sequencing Method. Guo, Bingfu,Guo, Yong,Hong, Huilong,Qiu, Li-Juan,Guo, Bingfu,Guo, Yong,Hong, Huilong,Qiu, Li-Juan,Hong, Huilong. 2016

[6]Effects of elevated air temperature on physiological characteristics of flag leaves and grain yield in rice. Liu, Qi-Hua,Wu, Xiu,Ma, Jia-Qing,Zhou, Xue-Biao,Li, Tian. 2013

[7]Detection of epistatic interactions of three QTLs for heading date in rice using single segment substitution lines. Ding, Han-Feng,Liu, Xu,Li, Run-Fang,Wang, Wen-Ying,Zhang, Y.,Zhang, Xiao-Dong,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song,Ding, Han-Feng.

[8]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[9]Application of silicon fertilizer affects nutritional quality of rice. Liu, Qihua,Zhou, Xuebiao,Sun, Zhaowen. 2017

[10]Frequency distribution of sensitivity of Ustilaginoidea virens to four EBI fungicides, prochloraz, difenoconazole, propiconazole and tebuconazole, and their efficacy in controlling rice false smut in Anhui Province of China. Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Yao, Jian,Li, Yun-Fei,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun. 2013

[11]Cultivar group of rice cultivated in Caoxieshan site (B.P.6000 similar to present) determined by the morphology of plant opals and its historical change. Wang, CL,Udatsu, T,Tang, LH,Zhou, JS,Zheng, YF,Sasaki, A,Yanagisawa, K,Fujiwara, H. 1998

[12]A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis. Zhou, Kunneng,Xia, Jiafa,Wang, Yuanlei,Ma, Tingchen,Li, Zefu. 2017

[13]Characterization and identification of cold tolerant near-isogenic lines in rice. Zhou, Lei,Hu, Guanglong,Pan, Yinghua,Zhang, Hongliang,Li, Jinjie,Li, Zichao,Zhou, Lei,Hu, Guanglong,Pan, Yinghua,Zhang, Hongliang,Li, Jinjie,Li, Zichao,Zeng, Yawen,Yang, Shuming,Zhou, Lei,You, Aiqing,Hu, Guanglong. 2012

[14]Molecular mapping of a pollen killer gene S29(t) in Oryza Glaberrima and co-linear analysis with S22 in O-Glumaepatula. Hu, Fengyi,Xu, Peng,Deng, Xianneng,Zhou, Jiawu,Li, Jing,Tao, Dayun. 2006

[15]Involvement of Polyamine Oxidase-Produced Hydrogen Peroxide during Coleorhiza-Limited Germination of Rice Seeds. Chen, Bing-Xian,Li, Wen-Yan,Gao, Yin-Tao,Chen, Zhong-Jian,Zhang, Wei-Na,Liu, Qin-Jian,Chen, Zhuang,Liu, Jun. 2016

[16]Reactive Oxygen Species Generated by NADPH Oxidases Promote Radicle Protrusion and Root Elongation during Rice Seed Germination. Li, Wen-Yan,Chen, Bing-Xian,Chen, Zhong-Jian,Gao, Yin-Tao,Chen, Zhuang,Liu, Jun. 2017

[17]Comparison of high-yield rice in tropical and subtropical environments - I. Determinants of grain and dry matter yields. Ying, JF,Peng, SB,He, QR,Yang, H,Yang, CD,Visperas, RM,Cassman, KG. 1998

[18]Genetic Diversity and Structure of New Inbred Rice Cultivars in China. Xu Qun,Wang Cai-hong,Yu Han-yong,Yuan Xiao-ping,Wang Yi-ping,Feng Yue,Tang Sheng-xiang,Wei Xing-hua,Chen Hong. 2012

[19]Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives. Wu, Tao,Yang, Chunyan,Ding, Baoxu,Feng, Zhiming,Wang, Qian,He, Jun,Jiang, Ling,Wan, Jianmin,Wan, Jianmin,Tong, Jianhua,Xiao, Langtao. 2016

[20]Driving the expression of RAA1 with a drought-responsive promoter enhances root growth in rice, its accumulation of potassium and its tolerance to moisture stress. Chen, Guang,Li, Chaolei,Gao, Zhenyu,Zhang, Yu,Zhu, Li,Hu, Jiang,Ren, Deyong,Qian, Qian,Chen, Guang,Xu, Guohua. 2018

作者其他论文 更多>>

微信小程序