Multi-omics unravel the compromised mucosal barrier function linked to aberrant mucin O-glycans in a pig model

文献类型： 外文期刊

第一作者： Xia, Bing

作者： Xia, Bing;Zhao, Xin;Xia, Bing;Zhong, Ruqing;Meng, Qingshi;Wu, Weida;Chen, Liang;Zhang, Hongfu;Zhao, Xin

作者机构：

关键词： Mucosal barrier function; Mucin O-glycans; Multi-omics; Truncated glycans

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 207 卷

页码：

收录情况： SCI

摘要： Early weaning stress (EWS) in piglets is associated with intestinal dysfunction. Here, utilizing a pig EWS model to mimic early-life stress (ELS) in humans, we investigated the mechanism of ELS-induced intestinal diseases through integrated multi-omics analyses of proteome, glycome, and microbiome. Our results demonstrated that EWS resulted in disrupted the ileal barrier integrity by reducing tight junction-related gene expression and interfering with cell-cell adhesion paralleled the increased proportion of pathogens such as Escherichia_Shigella and Helicobacter. Furthermore, Proteome data revealed that the accumulation of unfolded proteins and insufficient unfolded protein response (UPR) process caused by EWS led to ER stress. Data from proteome and glycome found that EWS induced aberrant mucin O-glycans, including truncated glycans, reduction in acidic glycans, and increased in fucosylated glycans. In addition, correlation test by taking fucose and inflammatory response into account suggested that enhancement of fucose expression might be a compensatory host response. Taken together, these results extend the comprehensive knowledge of the detrimental impacts and pathogenesis of EWS and help to provide intervention targets for ELS-induced intestinal diseases in the future.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序