Development of SSR Markers for and Fingerprinting of Walnut Genetic Resources

文献类型： 外文期刊

第一作者： Zhang, Suilin

作者： Zhang, Suilin;Li, Yang;Li, Yan;Zhang, Yunqi;Hao, Yanbin;Qi, Jianxun;Zhang, Suilin;Li, Yang;Li, Yan;Hou, Zhixia

作者机构：

关键词： walnut; nut phenotype; simple sequence repeats; breeding

期刊名称：FORESTS （ 影响因子：2.9； 五年影响因子：3.0 ）

ISSN：

年卷期： 2024 年 15 卷 3 期

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收录情况： SCI

摘要： Walnut is one of four major nuts in the world. China has abundant walnut germplasm resources, but there are still shortcomings in the identification of germplasm resources. This study used different walnut varieties as materials and developed 14 high-quality SSR molecular markers from 60 pairs of primers based on genome re-sequencing results. This study analyzed the genetic diversity of Chinese walnut genetic resources using 14 selected SSR markers. A total of 64 alleles were detected in 47 walnut resources, with an average of 4.571 alleles per locus. The variation range of polymorphism information content was 0.096 similar to 0.711, with an average value of 0.422. Cluster analysis, population genetic structure, and principal coordinate analysis divided 47 walnut resources into ordinary walnuts, Juglans hopeiensis, and Liaoyi 1. In addition, core SSR markers (Jr45, Jr40, Jr29, Jr35, and Jr11) were selected from 14 SSR markers, which were sufficient to distinguish 47 walnut resources. At the same time, 47 unique molecular fingerprints of walnuts were constructed using these core SSR markers. This study provides strong scientific support for rapid and efficient identification, germplasm innovation, and a variety of property protection of walnut germplasm.

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